The Influence of Stimulus Parameters on Contractions of Isolated Frog Muscle Fibres

Overview

Journal J Physiol

Specialty Physiology

Date 1969 Nov 1

PMID 5357251

Citations 11

Authors

R Rudel

S R Taylor

Affiliations

Soon will be listed here.

Abstract

1. Reversible changes in twitch and tetanus contractions of isolated frog muscle fibres were produced by varying the duration and strength of a transversely applied d.c. stimulus.2. When the stimulus strength was at least 1.1 times threshold the peak force of the contraction elicited was reduced by stimulus durations longer than 1 msec at 20 degrees C, and by durations longer than 3 msec at 2-5 degrees C.3. As the strength of a prolonged stimulus was progressively increased to 3 times rheobase or more, peak twitch and tetanus force declined to 40-70% of the amplitude with short (0.2-0.5 msec) stimuli. The time to peak of the twitch was slightly decreased and the first phase of relaxation was accelerated.4. Cine-micrography of fibres which were permitted to shorten under a light load revealed that the side of the fibre adjacent to the anode was unactivated under conditions which also reduced peak force output.5. With stimulus durations longer than 20 msec, peak force, time to peak, and relaxation time increased as the stimulus strength increased from 2.5 to 3 times rheobase. This might have been produced by a cathodal contracture following the action potential.6. The results are discussed in relation to previous studies of activation in striated muscle.

Citing Articles

Alternating bipolar field stimulation identifies muscle fibers with defective excitability but maintained local Ca(2+) signals and contraction.

Hernandez-Ochoa E, Vanegas C, Iyer S, Lovering R, Schneider M Skelet Muscle. 2016; 6:6.

PMID: 26855765 PMC: 4743112. DOI: 10.1186/s13395-016-0076-8.

Light diffraction studies of active muscles fibres as a function of sarcomere length.

Oba T, Baskin R, Lieber R J Muscle Res Cell Motil. 1981; 2(2):215-24.

PMID: 7263856 DOI: 10.1007/BF00711871.

Calcium transients in asymmetrically activated skeletal muscle fibers.

Trube G, Lopez J, Taylor S Biophys J. 1981; 36(3):491-507.

PMID: 6976801 PMC: 1327642. DOI: 10.1016/S0006-3495(81)84748-0.

Structural studies of the waves in striated muscle fibres shortened passively below their slack length.

Brown L, Gonzalez-Serratos H, HUXLEY A J Muscle Res Cell Motil. 1984; 5(3):273-92.

PMID: 6611351 DOI: 10.1007/BF00713108.

Intrinsic shortening speed of temperature-jump-activated intact muscle fibers. Effects of varying osmotic pressure with sucrose and KCl.

Gulati J, Babu A Biophys J. 1984; 45(2):431-45.

PMID: 6607750 PMC: 1434860. DOI: 10.1016/S0006-3495(84)84166-1.

References

HUXLEY A, Taylor R . Local activation of striated muscle fibres. J Physiol. 1958; 144(3):426-41. PMC: 1356788. DOI: 10.1113/jphysiol.1958.sp006111. View

Rudel R, Taylor S . Partial inhibition of contraction by prolongation of the transverse stimulating current. J Physiol. 1969; 202(2):69P-71P. View

Close R, Hoh J . The after-effects of repetitive stimulation on the isometric twitch contraction of rat fast skeletal muscle. J Physiol. 1968; 197(2):461-77. PMC: 1351810. DOI: 10.1113/jphysiol.1968.sp008570. View

Kelly E, FRY W . MUSCLE TWITCH TENSION--INFLUENCE OF ELECTRICAL STIMULATING CONDITIONS AT DIFFERENT TEMPERATURES. 3. ANALYSIS AND CORRELATION. Phys Med Biol. 1965; 10:251-60. DOI: 10.1088/0031-9155/10/2/309. View

Lee K, Ladinsky H, Choi S, Kasuya Y . Studies on the in vitro interaction of electrical stimulation and Ca++ movement in sarcoplasmic reticulum. J Gen Physiol. 1966; 49(4):689-715. PMC: 2195502. DOI: 10.1085/jgp.49.4.689. View

HUXLEY A, Peachey L . The maximum length for contraction in vertebrate straiated muscle. J Physiol. 1961; 156:150-65. PMC: 1359941. DOI: 10.1113/jphysiol.1961.sp006665. View

Katz B . Multiple response to constant current in frog's medullated nerve. J Physiol. 1936; 88(2):239-55. PMC: 1395266. DOI: 10.1113/jphysiol.1936.sp003435. View

COSTANTIN L, PODOLSKY R . Depolarization of the internal membrane system in the activation of frog skeletal muscle. J Gen Physiol. 1967; 50(5):1101-24. PMC: 2225702. DOI: 10.1085/jgp.50.5.1101. View

MASHIMA H, Washio H . The changes in membrane potential produced by alternating current or repetitive square pulses in the frog skeletal muscle fibres. Jpn J Physiol. 1968; 18(4):403-16. DOI: 10.2170/jjphysiol.18.403. View

10.

Sugi H, Ochi R . The mode of transverse spread of contraction initiated by local activation in single frog muscle fibers. J Gen Physiol. 1967; 50(9):2167-76. PMC: 2225770. DOI: 10.1085/jgp.50.9.2167. View

11.

Jenerick H . The relations between prepotential, resting potential, and latent period in frog muscle fibers. J Gen Physiol. 1956; 39(5):773-87. PMC: 2147565. DOI: 10.1085/jgp.39.5.773. View

12.

BLINKS J . INFLUENCE OF OSMOTIC STRENGTH ON CROSS-SECTION AND VOLUME OF ISOLATED SINGLE MUSCLE FIBRES. J Physiol. 1965; 177:42-57. PMC: 1357223. DOI: 10.1113/jphysiol.1965.sp007574. View

13.

Sugi H, Kosaka K . SUMMATION OF CONTRACTION IN SINGLE CRAYFISH MUSCLE FIBRES. Jpn J Physiol. 1964; 14:450-67. DOI: 10.2170/jjphysiol.14.450. View

14.

Kelly E, FRY W, Fry R . MUSCLE TWITCH TENSION--INFLUENCE OF ELECTRICAL STIMULATING CONDITIONS AT DIFFERENT TEMPERATURES. Phys Med Biol. 1964; 9:371-88. DOI: 10.1088/0031-9155/9/3/309. View

15.

BIRKS R, Davey D . Osmotic responses demonstrating the extracellular character of the sarcoplasmic reticulum. J Physiol. 1969; 202(1):171-88. PMC: 1351472. DOI: 10.1113/jphysiol.1969.sp008802. View

16.

HUXLEY A, Gordon A . Striation patterns in active and passive shortening of muscle. Nature. 1962; 193:280-1. DOI: 10.1038/193280b0. View

17.

Hartree W, Hill A . The nature of the isometric twitch. J Physiol. 1921; 55(5-6):389-411. PMC: 1405351. DOI: 10.1113/jphysiol.1921.sp001984. View

18.

GILSON Jr A, SCHOEPFLE G, Walker S . The time course of tension development in the muscle response. Ann N Y Acad Sci. 1947; 47(Art 6):697-714. DOI: 10.1111/j.1749-6632.1947.tb31733.x. View

19.

Sandow A, Isaacson A . Topochemical factors in potentiation of contraction by heavy metal cations. J Gen Physiol. 1966; 49(5):937-61. PMC: 2195525. DOI: 10.1085/jgp.49.5.937. View