Quantative Radioautographic Light and Electron Microscopic Analysis of the Localization of Monoamines in the Median Eminence of the Rat

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1979 Dec 1

PMID 535033

Citations 2

Authors

M A Belenky

V K Chetverukhin

A L POLENOV

Affiliations

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Abstract

Serotonin containing structures in the median eminence of the rat have been studied by quantitative light and electron microscopic radioautography following intraventricular infusion of tritiated 5-hydroxytryptophan. One hour after injection of the tracer the highest density of silver grains was recorded in the ependymal and external zones, especially in the lateral palisade zone. The proportion of labelled neurosecretory terminals was also larger in the lateral palisade zone (29%) as compared with the medial palisade zone (13%), although the mean number of developed silver grains per one terminal was higher in the latter. On the average, 16% of neurosecretory terminals sequestered radiolabelled 5-hydroxytryptophan in the external zone of the rat median eminence. It is suggested that serotonin, like catecholamines, is discharged from neurosecretory terminals localized in the external zone and via the portal circulation affects the function of the anterior pituitary. The sites of origin of serotoninergic structures of the median eminence as well as the possible role of monoamine (catecholamine and indolamine) neurohormones in a dual peptidergic and monoaminergic control of anterior pituitary functions are discussed.

Citing Articles

The hypothalamo-hypophysial system of hypophysectomized rats. II. Structure and ultrastructure of the median eminence.

POLENOV A, Belenky M Cell Tissue Res. 1981; 218(3):607-22.

PMID: 7261044 DOI: 10.1007/BF00210119.

The hypothalamo-hypophysial system of the lamprey, Lampetra fluviatilis L. III. High-resolution radioautography of monoaminergic structures in neurohemal regions.

Belenky M, Chetverukhin V, POLENOV A Cell Tissue Res. 1979; 204(2):333-42.

PMID: 317013 DOI: 10.1007/BF00234644.

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