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Studies on Insulin Biosynthesis. Subcellular Distribution of Leucine-H3 Radioactivity During Incubation of Goosefish Islet Tissue

Overview
Journal J Cell Biol
Specialty Cell Biology
Date 1966 Mar 1
PMID 5335636
Citations 10
Authors
G E BAUER
A W LINDALL Jr
P K Dixit
G Lester
A LAZAROW
Affiliations
Soon will be listed here.
Abstract

Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.

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