Effects of Ethacrynic Acid on Electrolyte and Fluid Transport by the Guinea Pig Gallbladder

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1979 Nov 1

PMID 530308

Citations 3

Authors

K U Petersen

K Heintze

L C Busch

O Heidenreich

Affiliations

Soon will be listed here.

Abstract

The effect of ethacrynic acid on fluid and electrolyte transport by the guinea pig gallbladder was investigated in vitro. 10-4M ethacrynic acid, applied to the serosal side, inhibited fluid and sodium chloride absorption. The reduction in salt absorption was accounted for by a 3 muEq/cm2h decrease in the unidirectional fluxes of Na and Cl from mucosa to serosa with no change in the fluxes from serosa to mucosa. Ethacrynic acid (10-4 M) had no effect on HCO3 - Cl exchange, PGE1-induced fluid secretion and inulin permeability. At 10-3 M, ethacrynic acid markedly increased both the serosa to mucosa fluxes of Na and Cl, and the inulin permeability. Examination by light and electron microscopy of gallbladder tissue treated with 10-3 M ethacrynic acid revealed large intracellular vacuoles and occasionally ruptured apical cell membranes. Only slight morphological changes were seen by 10-4 M ethacrynic acid with no changes in the controls and ouabain treated gallbladders. The effects of ethacrynic acid are remarkably different from those of furosemide which has been previously shown to inhibit only the HCO3 secretion leaving fluid and NaCl absorption unchanged.

Citing Articles

Relative effects of furosemide and ethacrynic acid on ion transport and energy metabolism in slices of rat kidney-cortex.

van Rossum G, Ernst S, Russo M Naunyn Schmiedebergs Arch Pharmacol. 1981; 317(1):90-6.

PMID: 7279013 DOI: 10.1007/BF00506264.

Effect of "high ceiling" diuretics on active salt transport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Correlation of chemical structure and inhibitory potency.

Schlatter E, Greger R, Weidtke C Pflugers Arch. 1983; 396(3):210-7.

PMID: 6844125 DOI: 10.1007/BF00587857.

Influence of serosal hydrostatic pressure on net water and electrolyte transport across the isolated rat colonic mucosa exposed to different secretagogues.

Karbach U, Wanitschke R Naunyn Schmiedebergs Arch Pharmacol. 1984; 327(4):336-41.

PMID: 6514016 DOI: 10.1007/BF00506246.

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