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Investigation into Rabbit Infusion Media for the Growth of Mycoplasma Gallisepticum Antigens for Inoculation into Rabbits

Overview
Journal J Hyg (Lond)
Specialty Public Health
Date 1971 Mar 1
PMID 5291753
Citations 1
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Abstract

Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.However, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.

Citing Articles

The influence of pH of the culture medium on the sensitivity of Mycoplasma gallisepticum antigens for use in certain serological tests.

Bradbury J, Jordan F J Hyg (Lond). 1971; 69(4):593-606.

PMID: 5002646 PMC: 2131049. DOI: 10.1017/s0022172400021872.

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