Ion Transport Underlying Metabolically Controlled Volume Changes of Isolated Mitochondria

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1970 Oct 1

PMID 5289016

Citations 6

Authors

S Izzard

H Tedeschi

Affiliations

Soon will be listed here.

Abstract

An earlier study has indicated that the swelling of isolated mitochondria induced by inorganic phosphate (P(i)) can be accounted for largely or completely by the accumulation of ions. In the present study, a similar uptake was shown to be induced by a wider range of P(i) concentrations. Addition of ADP, KCN, or 2,4-dinitrophenol initiates a shrinkage which can be accounted for by the efflux of ions. The results are consistent with the explanations that (a) P(i) induces a transport of ions and a concomitant osmotic swelling, and (b) the addition of substances which compete or interfere with the energy available for transport results in ion efflux and a corresponding mitochondrial shrinkage. The results are not consistent with proposals that the changes in the light scattered by mitochondrial suspensions with alterations in metabolic states reflect a mechanochemical coupling phenomenon.

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References

Azzi A, Azzone G . Swelling and shrinkage phenomena in liver mitochondria. II. Low amplitude swelling-shrinkage cycles. Biochim Biophys Acta. 1965; 105(2):265-78. DOI: 10.1016/s0926-6593(65)80151-5. View

Tupper J, Tedeschi H . Mitochondrial membrane potentials measured with microelectrodes: probable ionic basis. Science. 1969; 166(3912):1539-40. DOI: 10.1126/science.166.3912.1539. View

Weinbach E, GARBUS J, SHEFFIELD H . Morphology of mitochondria in the coupled, uncoupled and recoupled states. Exp Cell Res. 1967; 46(1):129-43. DOI: 10.1016/0014-4827(67)90415-6. View

Hackenbrock C . Ultrastructural bases for metabolically linked mechanical activity in mitochondria. I. Reversible ultrastructural changes with change in metabolic steady state in isolated liver mitochondria. J Cell Biol. 1966; 30(2):269-97. PMC: 2107001. DOI: 10.1083/jcb.30.2.269. View

Packer L, Utsumi R, Mustafa M . Oscillatory states of mitochondria. 1. Electron and energy transfer pathways. Arch Biochem Biophys. 1966; 117(2):381-93. DOI: 10.1016/0003-9861(66)90426-7. View

Utsumi K, Packer L . Oscillatory states of mitochondria. II. Factors controlling period and amplitude. Arch Biochem Biophys. 1967; 120(2):404-12. DOI: 10.1016/0003-9861(67)90257-3. View

Tupper J, Tedeschi H . Observations on low amplitude Ca2+-induced swelling in mitochondria. Life Sci. 1967; 6(19):2021-8. DOI: 10.1016/0024-3205(67)90220-2. View

Deamer D, Utsumi K, Packer L . Oscillatory states of mitochondria. 3. Ultrastructure of trapped conformational states. Arch Biochem Biophys. 1967; 121(3):641-51. DOI: 10.1016/0003-9861(67)90049-5. View

Harris R, Penniston J, Asai J, Green D . The conformational basis of energy conservation in membrane systems. II. Correlation between conformational change and functional states. Proc Natl Acad Sci U S A. 1968; 59(3):830-7. PMC: 224758. DOI: 10.1073/pnas.59.3.830. View

10.

Penniston J, Harris R, Asai J, Green D . The conformational basis of energy transformations in membrane systems. I. Conformational changes in mitochondria. Proc Natl Acad Sci U S A. 1968; 59(2):624-31. PMC: 224718. DOI: 10.1073/pnas.59.2.624. View

11.

Hackenbrock C . Ultrastructural bases for metabolically linked mechanical activity in mitochondria. II. Electron transport-linked ultrastructural transformations in mitochondria. J Cell Biol. 1968; 37(2):345-69. PMC: 2107416. DOI: 10.1083/jcb.37.2.345. View

12.

Green D, Asai J, Harris R, Penniston J . Conformational basis of energy transformations in membrane systems. 3. Configurational changes in the mitochondrial inner membrane induced by changes in functional states. Arch Biochem Biophys. 1968; 125(2):684-705. DOI: 10.1016/0003-9861(68)90626-7. View

13.

Packer L, Wrigglesworth J, Fortes P, PRESSMAN B . Expansion of the inner membrane compartment and its relation to mitochondrial volume and ion transport. J Cell Biol. 1968; 39(2):382-91. PMC: 2107527. DOI: 10.1083/jcb.39.2.382. View

14.

BLONDIN G, Vail W, Green D . The mechanism of mitochondrial swelling. II. Pseudoenergized swelling in the presence of alkali metal salts. Arch Biochem Biophys. 1969; 129(1):158-72. DOI: 10.1016/0003-9861(69)90162-3. View

15.

Harris E, PRESSMAN B . The direction of polarity of the mitochondrial trans-membrane potential. Biochim Biophys Acta. 1969; 172(1):66-70. DOI: 10.1016/0005-2728(69)90092-9. View

16.

Stoner C, SIRAK H . Passive induction of the "energized-twisted" conformational state in bovine heart mitochondria. Biochem Biophys Res Commun. 1969; 35(1):59-66. DOI: 10.1016/0006-291x(69)90482-3. View

17.

SORDAHL L, Blailock Z, Kraft G, Schwartz A . The possible relationship between ultrastructure and biochemical state of heart mitochondria. Arch Biochem Biophys. 1969; 132(2):404-15. DOI: 10.1016/0003-9861(69)90382-8. View

18.

BLONDIN G, Green D . Mechanism of mitochondrial swelling. 3. Two forms of energized swelling. Arch Biochem Biophys. 1969; 132(2):509-23. DOI: 10.1016/0003-9861(69)90395-6. View

19.

Asai J, BLONDIN G, Vail W, Green D . The mechanism of mitochondrial swelling. IV. Configurational changes during swelling of beef heart mitochondria. Arch Biochem Biophys. 1969; 132(2):524-44. DOI: 10.1016/0003-9861(69)90396-8. View

20.

Harris R, Asbell M, Asai J, Jolly W, Green D . The conformational basis of energy transduction in membrane systems. V. Measurement of configurational changes by light scattering. Arch Biochem Biophys. 1969; 132(2):545-60. DOI: 10.1016/0003-9861(69)90397-x. View