An Ultrastructural and Cytochemical Study of Neuromuscular Junctions in Echinoderms

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1975 Sep 29

PMID 52643

Citations 4

Authors

H Dolder

Affiliations

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Abstract

The ultrastructure of neuromuscular junctions in various organs of a starfish and a holothurian was studied. All neurons were found to contain large (100-250 nm), dense-core vesicles. Cytochemical tests for acetylcholinesterase were negative for these neurons. The presence of proteinaceous neurosecretory material was contested by enzymatic digestion (pepsin) which did not attack the dense-core vesicles, as well as by incubation in phosphotungstic acid (PTA) which did not stain these structures. Staining with dichromate produced a positive reaction for 5-hydroxytryptamine. In the absence of synaptic modifications even after specific staining with PTA, the transmission of nervous impulses is effectuated through exocytosis of 5-hydroxytryptamine at nerve endings in the muscle tissue.

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Ultrastructural, fluorescence microscopic and microfluorimetric study of the innervation of the axial complex in the sea urchin, Sphaerechinus granularis (lam.).

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An ultrastructural study of the dermal papulae of the starfish, Asterias rubens, with special reference to innervation of the muscles.

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