Reversible Transformation of Fibrous Collagen to a Soluble State in Vivo

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1969 Mar 1

PMID 5257013

Citations 3

Authors

L Klein

Affiliations

Soon will be listed here.

Abstract

Metabolic pathways of pre-existing and newly synthesized collagen fractions from skin and induced connective tissue were studied in rats that had been labeled with (3)H-L-proline 6 and 20 weeks previously. The distribution of specific radioactivity was determined for soluble and insoluble collagens. Multiple extractions of neutral salt-soluble and citrate-soluble collagen demonstrated constant specific radioactivities which indicated the achievement of an isotopic steady state. Citrate-soluble collagen was four times more radioactive than neutral salt-soluble collagen, which indicated a large difference in biological age (months) between fractions. Different levels of specific radioactivity in collagen fractions from implanted sponge indicated that both pre-existing (citrate-soluble) and newly synthesized collagen (salt-soluble) contributed to the insoluble collagen of induced connective tissue in normal and hypophysectomized rats. The isotopic data indicated that pre-existing fibrous collagen could be solubilized in vivo and translocated from skin to sponge via citrate-soluble collagen.

Citing Articles

Influence of streptozotocin and alloxan induced diabetes on the metabolism of dermal collagen in albino rats.

Mohanam S, Bose S Acta Diabetol Lat. 1981; 18(3):251-8.

PMID: 6458190 DOI: 10.1007/BF02047897.

Influence of prednisolone and L-thyroxine on the changes in collagen metabolism in rats with adjuvant-induced arthritis.

Kuberasampath T, BOSE S Agents Actions. 1979; 9(5-6):502-9.

PMID: 546173 DOI: 10.1007/BF01968119.

Mechanism of inhibition of collagen crosslinking by penicillamine.

Nimni M Proc R Soc Med. 1977; 70 Suppl 3:65-72.

PMID: 122677 PMC: 1543564.

References

Nikkari T, KULONEN E . Studies in experimental lathyrism. II. On the properties of collagen. Biochem Pharmacol. 1962; 11:931-6. DOI: 10.1016/0006-2952(62)90142-9. View

HENRIQUES O, HENRIQUES S, Neuberger A . Quantitative aspects of glycine metabolism in the rabbit. Biochem J. 1955; 60(3):409-24. PMC: 1215716. DOI: 10.1042/bj0600409. View

JACKSON D . Connective tissue growth stimulated by carrageenin. I. The formation and removal of collagen. Biochem J. 1957; 65(2):277-84. PMC: 1199866. DOI: 10.1042/bj0650277. View

POPENOE E, VAN SLYKE D . The formation of collagen hydroxylysine. J Biol Chem. 1962; 237:3491-4. View

REITHEL F . THE DISSOCIATION AND ASSOCIATION OF PROTEIN STRUCTURES. Adv Protein Chem. 1963; 18:123-226. DOI: 10.1016/s0065-3233(08)60269-7. View

Bailey A . Intermediate labile intermolecular crosslinks in collagen fibres. Biochim Biophys Acta. 1968; 160(3):447-53. DOI: 10.1016/0005-2795(68)90216-x. View

Klein L, Weiss P . Induced connective tissue metabolism in vivo: reutilization of pre-existing collagen. Proc Natl Acad Sci U S A. 1966; 56(1):277-84. PMC: 285707. DOI: 10.1073/pnas.56.1.277. View

Gross J . Studies on the formation of collagen. II. The influence of growth rate on neutral salt extracts of guinea pig dermis. J Exp Med. 1958; 107(2):265-77. PMC: 2136799. DOI: 10.1084/jem.107.2.265. View

BALLOU J, Thompson R . Studies of metabolic turnover with tritium as a tracer. V. The predominantly non-dynamic state of body constituents in the rat. J Biol Chem. 1956; 223(2):795-809. View

10.

STETTEN M . Some aspects of the metabolism of hydroxyproline, studied with the aid of isotopic nitrogen. J Biol Chem. 1949; 181(1):31-7. View

11.

Neuberger A, SLACK H . The metabolism of collagen from liver, bone, skin and tendon in the normal rat. Biochem J. 1953; 53(1):47-52. PMC: 1198099. DOI: 10.1042/bj0530047. View

12.

Klein L . Use of chronically labeled animals in the study of a "metabolically inert" protein. Adv Tracer Methodol. 1968; 4:215-26. DOI: 10.1007/978-1-4684-7532-6_16. View

13.

Klein L, Weiss P . Reutilization of mature collagen in vivo. Biochem Biophys Res Commun. 1965; 21(4):311-7. DOI: 10.1016/0006-291x(65)90194-4. View

14.

Weiss P, Klein L . The quantitative relationship of urinary peptide hydroxyproline excretion to collagen degradation. J Clin Invest. 1969; 48(1):1-10. PMC: 322186. DOI: 10.1172/JCI105957. View

15.

Meselson M, Stahl F . THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958; 44(7):671-82. PMC: 528642. DOI: 10.1073/pnas.44.7.671. View

16.

OREKHOVITCH V, SHPIKITER V . Procollagens. Science. 1958; 127(3311):1371-6. DOI: 10.1126/science.127.3311.1371. View

17.

JACKSON D, Bentley J . On the significance of the extractable collagens. J Biophys Biochem Cytol. 1960; 7:37-42. PMC: 2224869. DOI: 10.1083/jcb.7.1.37. View

18.

GONZALEZ CADAVID N, Denduchis B, MANCINI R . Soluble collagens in normal rat skin, from embryo to adulthood. Lab Invest. 1963; 12:598-605. View

19.

Davison A, Dobbing J . Metabolic stability of body constituents. Nature. 1961; 191:844-8. DOI: 10.1038/191844a0. View

20.

CHULKOVA T, OREKHOVICH V . [Effect of somatotropic hormone on incorporation of radioactive proline into procollagen]. Vopr Med Khim. 1965; 11(6):76-7. View