Evidence for One Functional Phenylalanyl-tRNA Binding Site on the 30S Ribosomal Subunit

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1969 Feb 1

PMID 5256228

Citations 1

Authors

K Igarashi

A Kaji

Affiliations

Soon will be listed here.

Abstract

NH(2) terminal analysis of polyphenylalanine formed from 30S subunit-bound (14)C-phenylalanyl-tRNA suggests that there is only one site in a 30S subunit for specific binding of phenylalanyl-tRNA. Assuming that no movement of the bound phenylalanyl-tRNA takes place during association of 30S subunits with 50S ribosomal subunits, the binding site of the 30S ribosomal subunit corresponds to site 2 (-NH(2) side with respect to the growing polypeptide chain) of the two binding sites of 70S ribosomes.

Citing Articles

Dissociation of N(2) Gas-induced Monomeric Ribosomes and Functioning of the Derived Subunits in Protein Synthesis in Pea.

Lin C, Key J Plant Physiol. 1971; 48(5):547-52.

PMID: 16657836 PMC: 396904. DOI: 10.1104/pp.48.5.547.

References

Takeda Y, SUZUKA I, Kaji A . Comparative studies on specific and nonspecific binding of transfer ribonucleic acid to ribosomes. J Biol Chem. 1968; 243(6):1075-81. View

Pestka S, Nirenberg M . Regulatory mechanisms and protein synthesis. X. Codon recognition on 30 S ribosomes. J Mol Biol. 1966; 21(1):145-71. DOI: 10.1016/0022-2836(66)90085-4. View

Igarashi K, Kaji A . On the nature of two ribosomal sites for specific sRNA binding. Proc Natl Acad Sci U S A. 1967; 58(5):1971-6. PMC: 223892. DOI: 10.1073/pnas.58.5.1971. View

Nakamoto T . The initial phase in the polyuridylic acid-directed polymerization of phenylalanine. J Biol Chem. 1967; 242(19):4534-7. View

Martin R, Ames B . THE EFFECT OF POLYAMINES AND OF POLY U SIZE ON PHENYLALANINE INCORPORATION. Proc Natl Acad Sci U S A. 1962; 48(12):2171-8. PMC: 221140. DOI: 10.1073/pnas.48.12.2171. View

Goldberg I, Mitsugi K . Inhibition by sparsomycin and other antibiotics of the puromycin-induced release of polypeptide from ribosomes. Biochemistry. 1967; 6(2):383-91. DOI: 10.1021/bi00854a003. View

Iwasaki K, Sabol S, Wahba A, Ochoa S . Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes. Arch Biochem Biophys. 1968; 125(2):542-7. DOI: 10.1016/0003-9861(68)90612-7. View

Revel M, Herzberg M, BECAREVIC A, Gros F . Role of protein factor in the functional binding of ribosomes to natural messenger RNA. J Mol Biol. 1968; 33(1):231-49. DOI: 10.1016/0022-2836(68)90291-x. View

SUZUKA I, Kaji H, Kaji A . Binding of specific sRNA to 30S subunits--comparison with the binding to 70S ribosomes. Biochem Biophys Res Commun. 1965; 21(2):187-93. DOI: 10.1016/0006-291x(65)90107-5. View

10.

Nirenberg M, Leder P . RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964; 145(3639):1399-407. DOI: 10.1126/science.145.3639.1399. View

11.

Vazquez D, MONRO R . Effects of some inhibitors of protein synthesis on the binding of aminoacyl tRNA to ribosomal subunits. Biochim Biophys Acta. 1967; 142(1):155-73. DOI: 10.1016/0005-2787(67)90524-2. View

12.

Kaji H, SUZUKA I, Kaji A . Binding of specific soluble ribonucleic acid to ribosomes. Binding of soluble ribonucleic acid to the template-30 S subunits complex. J Biol Chem. 1966; 241(6):1251-6. View

13.

KURIKI Y, Kaji A . Factor- and guanosine 5'-triphosphate-dependent release of deacylated transfer RNA from 70S ribosomes. Proc Natl Acad Sci U S A. 1968; 61(4):1399-405. PMC: 225269. DOI: 10.1073/pnas.61.4.1399. View

14.

SUZUKA I, Kaji H, Kaji A . Binding of specific sRNA to 30S ribosomal subunits: effect of 50S ribosomal subunits. Proc Natl Acad Sci U S A. 1966; 55(6):1483-90. PMC: 224348. DOI: 10.1073/pnas.55.6.1483. View

15.

Nathans D, LIPMANN F . Amino acid transfer from aminoacyl-ribonucleic acids to protein on ribosomes of Escherichia coli. Proc Natl Acad Sci U S A. 1961; 47:497-504. PMC: 221479. DOI: 10.1073/pnas.47.4.497. View