Studies on the High-mobility-group Non-histone Proteins from Hen Oviduct

Overview

Journal Biochem J

Specialty Biochemistry

Date 1979 Sep 1

PMID 518542

Citations 5

Authors

C S Teng

G K Andrews

C T TENG

Affiliations

Soon will be listed here.

Abstract

Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, -2, -3, -14 and -17, which are equivalent to the classification of calf thymus HMG proteins. Hen oviduct proteins HMG-1 and -2 were individually isolated by HCIO4.extraction and CM-Sephadex chromatographic separation. Their mol.wts. were determined as 28 000 and 27 000, respectively. The proteins have a high content of acidic and basic amino acids. The association of proteins HMG-1 and -2 with the genome of hen oviduct nuclei was probed by a limited digestion with nucleases. Hen oviduct nuclei were incubated with deoxyribonuclease I or micrococcal nuclease until 10% of the DNA was digested. The nuclear suspension was centrifuged and the contents of proteins HMG-1 and -2 in the supernatant and sediment fractions were analysed by polyacrylamide-gel electrophoresis. HMG proteins were found to be preferentially released by micrococcal-nuclease digestion rather than by deoxyribonuclease I.

Citing Articles

Immuno-biochemical studies of a non-histone chromosomal protein in embryonic and mature chick oviduct.

TENG C, Teng C Biochem J. 1980; 185(1):169-75.

PMID: 6769427 PMC: 1161280. DOI: 10.1042/bj1850169.

Changes in quantities of high-mobility-group protein 1 in oviduct cellular fractions after oestrogen stimulation.

TENG C, Teng C Biochem J. 1981; 198(1):85-90.

PMID: 6459782 PMC: 1163213. DOI: 10.1042/bj1980085.

Analysis of putative high-mobility-group (HMG) proteins in neuronal and glial nuclei from rabbit brain.

Greenwood P, Silver J, Brown I Neurochem Res. 1981; 6(6):673-9.

PMID: 6456426 DOI: 10.1007/BF00963883.

Heterogeneity of proteins resembling high-mobility-group protein HMG-T in trout testes nuclei.

Brown E, Goodwin G, Mayes E, HASTINGS J, JOHNS E Biochem J. 1980; 191(2):661-4.

PMID: 6453585 PMC: 1162261. DOI: 10.1042/bj1910661.

Detection of monoclonal antibody to high-mobility-group protein 17 from chick oviduct.

Teng C, TENG C, Chan T Biochem J. 1982; 203(2):471-6.

PMID: 6214252 PMC: 1158252. DOI: 10.1042/bj2030471.

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