Enzymic Iodination. A Probe for Accessible Surface Proteins of Normal and Neoplastic Lymphocytes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1971 Oct 1

PMID 5131013

Citations 114

Authors

J J Marchalonis

R E Cone

V Santer

Affiliations

Soon will be listed here.

Abstract

1. Radioactive iodide was covalently bound to living cells from normal mouse spleen and a variety of lymphoid tumours by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. About 3x10(5)-6x10(5) molecules of [(125)I]iodide/cell could be incorporated without affecting cell viability. 3. Electron-micrographic radioautography showed that the radioactive label was associated with the outer surfaces of the cells. 4. Radioiodinated proteins were solubilized in 9m-urea-0.2m-mercaptoethanol and analysed by gel-filtration and disc electrophoresis. 5. Comparison of distinct tumour lines by disc electrophoresis showed qualitative and quantitative differences in protein distribution patterns.

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References

McCONAHEY P, Dixon F . A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966; 29(2):185-9. DOI: 10.1159/000229699. View

Hogg D, Jago G . The antibacterial action of lactoperoxidase. The nature of the bacterial inhibitor. Biochem J. 1970; 117(4):779-90. PMC: 1179031. DOI: 10.1042/bj1170779. View

Reisfeld R, Kahan B . Biological and chemical characterization of human histocompatibility antigens. Fed Proc. 1970; 29(6):2034-40. View

Morrison M, Hamilton H, Stotz E . The isolation and purification of lactoperoxidase by ion exchange chromatography. J Biol Chem. 1957; 228(2):767-76. View

Eisen H, Kern M, NEWTON W, Helmreich E . A study of the distribution of 2,4-dinitrobenzene sensitizers between isolated lymph node cells and extracellular medium in relation to induction of contact skin sensitivity. J Exp Med. 1959; 110(2):187-206. PMC: 2136994. DOI: 10.1084/jem.110.2.187. View

Lefkowitz R, Roth J, Pastan I . Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma. Science. 1970; 170(3958):633-5. DOI: 10.1126/science.170.3958.633. View

Berg H . Sulfanilic acid diazonium salt: a label for the outside of the human erythrocyte membrane. Biochim Biophys Acta. 1969; 183(1):65-78. DOI: 10.1016/0005-2736(69)90130-8. View

Reisfeld R, Kahan B . Transplantation antigens. Adv Immunol. 1970; 12:117-200. DOI: 10.1016/s0065-2776(08)60169-8. View

Nathenson S, Davies D . Solubilization and partial purification of mouse histocompatibility antigens from a membranous lipoprotein fraction. Proc Natl Acad Sci U S A. 1966; 56(2):476-83. PMC: 224397. DOI: 10.1073/pnas.56.2.476. View

10.

Parish C, Marchalonis J . A simple and rapid acrylamide gel method for estimating the molecular weights of proteins and protein subunits. Anal Biochem. 1970; 34(2):436-50. DOI: 10.1016/0003-2697(70)90128-4. View

11.

Salpeter M, Bachmann L . AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST. J Cell Biol. 1964; 22:469-77. PMC: 2106457. DOI: 10.1083/jcb.22.2.469. View

12.

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

13.

Marchalonis J, Edelman G . Phylogenetic origins of antibody structure. I. Multichain structure of immunoglobulins in the smooth dogfish (Mustelus canis). J Exp Med. 1965; 122(3):601-18. PMC: 2138074. DOI: 10.1084/jem.122.3.601. View

14.

Marchialonis J, Edelman G . Isolation and characterization of a hemagglutinin from Limulus polyphemus. J Mol Biol. 1968; 32(2):453-65. DOI: 10.1016/0022-2836(68)90022-3. View

15.

Byrt P, Ada G . An in vitro reaction between labelled flagellin or haemocyanin and lymphocyte-like cells from normal animals. Immunology. 1969; 17(4):503-16. PMC: 1455856. View

16.

Perper R, Zee T, Mickelson M . Purification of lymphocytes and platelets by gradient centrifugation. J Lab Clin Med. 1968; 72(5):842-8. View

17.

Edelman G, Gall W . The antibody problem. Annu Rev Biochem. 1969; 38:415-66. DOI: 10.1146/annurev.bi.38.070169.002215. View

18.

Ada G, Nossal G, Pye J . ANTIGENS IN IMMUNITY. III. DISTRIBUTION OF IODINATED ANTIGENS FOLLOWING INJECTION INTO RATS VIA THE HIND FOOTPADS. Aust J Exp Biol Med Sci. 1964; 42:295-310. View

19.

Marchalonis J, Nossal G . Electrophoretic analysis of antibody produced by single cells. Proc Natl Acad Sci U S A. 1968; 61(3):860-7. PMC: 305407. DOI: 10.1073/pnas.61.3.860. View

20.

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View