Growth of Aerobacter Aerogenes on D-arabinose: Origin of the Enzyme Activities

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Oct 1

PMID 5122807

Citations 11

Authors

E J Oliver

R P Mortlock

Affiliations

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Abstract

Mutants of Aerobacter aerogenes can be selected which are capable of utilizing d-arabinose as a sole source of carbon and energy for growth. Mutants can also be selected which are capable of using l-xylose. The mutational event permitting the utilization of d-arabinose results in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the first reaction in the degradation of d-arabinose: the isomerization of d-arabinose to d-ribulose. No other type of mutation appears to be required to initiate growth on d-arabinose, and wild-type cells induced for the enzymes of the l-fucose catabolic pathway are capable of growth on d-arabinose. The first reaction in the catabolism of l-xylose, the isomerization of l-xylose to l-xylulose, also appears to be catalyzed by constitutively synthesized l-fucose isomerase.

Citing Articles

Molecular cloning of the Escherichia coli B L-fucose-D-arabinose gene cluster.

Elsinghorst E, Mortlock R J Bacteriol. 1994; 176(23):7223-32.

PMID: 7961494 PMC: 197110. DOI: 10.1128/jb.176.23.7223-7232.1994.

Directed evolution of a second xylitol catabolic pathway in Klebsiella pneumoniae.

Doten R, Mortlock R J Bacteriol. 1984; 159(2):730-5.

PMID: 6378891 PMC: 215706. DOI: 10.1128/jb.159.2.730-735.1984.

Metabolism of D-arabinose by Aerobacter aerogenes: purification of the isomerase.

Oliver E, Mortlock R J Bacteriol. 1971; 108(1):293-9.

PMID: 5122810 PMC: 247065. DOI: 10.1128/jb.108.1.293-299.1971.

Regulation of D-xylose and D-arabitol catabolism by Aerobacter aerogenes.

Wilson B, Mortlock R J Bacteriol. 1973; 113(3):1404-11.

PMID: 4734863 PMC: 251711. DOI: 10.1128/jb.113.3.1404-1411.1973.

Evolution of L-1, 2-propanediol catabolism in Escherichia coli by recruitment of enzymes for L-fucose and L-lactate metabolism.

Cocks G, Aguilar T, Lin E J Bacteriol. 1974; 118(1):83-8.

PMID: 4595205 PMC: 246642. DOI: 10.1128/jb.118.1.83-88.1974.

References

Anderson R, Wood W . Pathway of L-xylose and L-lyxose degradation in Aerobacter aerogenes. J Biol Chem. 1962; 237:296-303. View

Anderson R, Wood W . Purification and properties of L-xylulokinase. J Biol Chem. 1962; 237:1029-33. View

Oliver E, Bisson T, Leblanc D, Mortlock R . D-Ribulose production by a mutant of Aerobacter aerogens. Anal Biochem. 1969; 27(2):300-5. DOI: 10.1016/0003-2697(69)90036-0. View

Bisson T, Oliver E, Mortlock R . Regulation of pentitol metabolism by aerobacter aerogenes. II. Induction of the ribitol pathway. J Bacteriol. 1968; 95(3):932-6. PMC: 252112. DOI: 10.1128/jb.95.3.932-936.1968. View

Camyre K, Mortlock R . Growth of Aerobacter aerogenes on D-arabinose and L-xylose. J Bacteriol. 1965; 90(4):1157-8. PMC: 315791. DOI: 10.1128/jb.90.4.1157-1158.1965. View

NEISH A, SIMPSON F . The anaerobic dissimilation of d-glucose-1-C14, d-arabinose-1-C14, and 1-arabinose-1-C14 by Aerobacter aerogenes. Can J Biochem Physiol. 1954; 32(3):147-53. View

Green M, COHEN S . Enzymatic conversion of L-fucose to L-fuculose. J Biol Chem. 1956; 219(2):557-68. View

Wood W . Carbohydrate metabolism. Annu Rev Biochem. 1966; 35:521-58. DOI: 10.1146/annurev.bi.35.070166.002513. View

ENGLESBERG E, Irr J, Power J, Lee N . Positive control of enzyme synthesis by gene C in the L-arabinose system. J Bacteriol. 1965; 90(4):946-57. PMC: 315760. DOI: 10.1128/jb.90.4.946-957.1965. View

10.

GHALAMBOR M, Heath E . The metabolism of L-fucose. II. The enzymatic cleavage of L-fuculose 1-phosphate. J Biol Chem. 1962; 237:2427-33. View

11.

Bisson T, Mortlock R . Regulation of pentitol metabolism by Aerobacter aerogenes. I. Coordinate control of ribitol dehydrogenase and D-ribulokinase activities. J Bacteriol. 1968; 95(3):925-31. PMC: 252111. DOI: 10.1128/jb.95.3.925-931.1968. View

12.

Anderson R, Allison D . PURIFICATION AND CHARACTERIZATION OF D-LYXOSE ISOMERASE. J Biol Chem. 1965; 240:2367-72. View

13.

Heath E, GHALAMBOR M . The metabolism of L-fucose. I. The purification and properties of L-fuculose kinase. J Biol Chem. 1962; 237:2423-6. View

14.

Mortlock R, FOSSITT D, Petering D, Wood W . METABOLISM OF PENTOSES AND PENTITOLS BY AEROBACTER AEROGENES. 3. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF PENITOL DEHYDROGENASES AND PENTULOKINASES. J Bacteriol. 1965; 89:129-35. PMC: 315559. DOI: 10.1128/jb.89.1.129-135.1965. View

15.

DISCHE Z, Borenfreund E . A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem. 1951; 192(2):583-7. View

16.

Cohen S . Studies on D-ribulose and its enzymatic conversion to D-arabinose. J Biol Chem. 1953; 201(1):71-84. View