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The Purification and Properties of Microsomal Palmitoyl-coenzyme A Synthetase

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Journal Biochem J
Specialty Biochemistry
Date 1971 Apr 1
PMID 5118106
Citations 36
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Abstract

The isolation and purification of palmitoyl-CoA synthetase from rat liver microsomes is described. Several methods suitable for enzyme assay are described. The general properties and kinetic parameters of the purified enzyme were determined and are discussed in relationship to microsomal fatty acid activation.

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References
1.
Kornberg A, PRICER Jr W . Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids. J Biol Chem. 1953; 204(1):329-43. View

2.
Vignais P, Zabin I . Synthesis and properties of palmityl adenylate, palmityl coenzyme A, and palmityl glutathione. Biochim Biophys Acta. 1958; 29(2):263-9. DOI: 10.1016/0006-3002(58)90183-5. View

3.
Vignais P, GALLAGHER C, Zabin I . Activation and oxidation of long chain fatty acids by rat brain. J Neurochem. 1958; 2(2-3):283-7. DOI: 10.1111/j.1471-4159.1958.tb12375.x. View

4.
Clarke J . SIMPLIFIED "DISC" (POLYACRYLAMIDE GEL) ELECTROPHORESIS. Ann N Y Acad Sci. 1964; 121:428-36. DOI: 10.1111/j.1749-6632.1964.tb14214.x. View

5.
Ailhaud G, Sarda L, DESNUELLE P . [Formation of hydroxamates of long chain fatty acids by a subcellular fraction of intestinal mucosa]. Biochim Biophys Acta. 1962; 59:261-72. DOI: 10.1016/0006-3002(62)90174-9. View