Sodium and Calcium Localization in Cells and Tissues by Precipitation with Antimonate: a Quantitative Study

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1979 Oct 1

PMID 511599

Citations 9

Authors

F van Iren

P Van der Duyn Schouten

P Boers-van der Sluijs

W C de Bruijn

Affiliations

Soon will be listed here.

Abstract

Komnick's antimonate technique, which was devised to localize Na+ in cells and tissues, was studied quantitatively. Some modifications, as well as its application to Ca2+ localization, were also investigated. We combined measurements of Na+ and Ca2+ retention in plant roots during the various procedures, electron microscopy, autoradiography, and semiquantitative X-ray microanalysis. We were able to show that (at least in barley roots) antimonate does not precipitate at all with Na+, irrespective of the Na+ content of the tissue or the method of antimonate application. (Even during precipitative freeze dissolution or after freeze drying, no Na+ is precipitated.) By means of Komnick's antimonate technique Ca2+ is trapped within the tissue, but only after serious dislocation. Perspectives for reliable localization of diffusible ions in cells and tissues, by precipitation simultaneously with conventional fixations, are bad.

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