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Increased Binding of Ethidium Bromide to Polynucleotide Duplexes Containing Mismatched Bases

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1979 Oct 25
PMID 503848
Citations 3
Authors
D C Helfgott
N R Kallenbach
Affiliations
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Abstract

The binding of the intercalating dye ethidium bromide to a series of synthetic polynucleotide duplexes containing varying concentrations of mismatched bases has been measured by fluorescence titration. The dye binds more strongly to duplexes with mismatches, the estimated increase in affinity being twenty-fold for the series of molecules poly (I).poly (C,Ax) with x denoting the mole fraction of mismatched A residues in the C strand. The results are consistent with one requirement of the Streisinger model for frameshift mutagenesis, namely that frameshifting agents can function by stablizing mismatched transient intermediates in DNA.

Citing Articles

Characterization of imperfect DNA duplexes containing unpaired bases and non-Watson-Crick base pairs.

Evans D, Morgan A Nucleic Acids Res. 1986; 14(10):4267-80.

PMID: 3714476 PMC: 339860. DOI: 10.1093/nar/14.10.4267.

Base specificity in the interaction of ethidium with synthetic polyribonucleotides.

Babayan Y, Manzini G, Xodo L, Quadrifoglio F Nucleic Acids Res. 1987; 15(14):5803-12.

PMID: 2441360 PMC: 306024. DOI: 10.1093/nar/15.14.5803.

Self-catalyzed cyclization of the intervening sequence RNA of Tetrahymena: inhibition by methidiumpropyl.EDTA and localization of the major dye binding sites.

Tanner N, Cech T Nucleic Acids Res. 1985; 13(21):7759-79.

PMID: 2415924 PMC: 322085. DOI: 10.1093/nar/13.21.7759.

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