In Vivo Release of Endogenous GABA in the Cat Hypothalamus

Overview

Journal Naunyn Schmiedebergs Arch Pharmacol

Specialty Pharmacology

Date 1979 Aug 1

PMID 503246

Citations 12

Authors

H Dietl

A Philippu

Affiliations

Soon will be listed here.

Abstract

The posterior hypothalamus of anaesthetized cats was superfused with artificial cerebrospinal fluid through a push-pull cannula and the release of endogenous GABA from the hypothalamus into the superfusate was studied. The resting release of GABA varied rhythmically, since phases of high rate of release were separated from each other by phases of low rate of release. The time interval between two adjacent phases of high rate of release was about 70 min. Electrical stimulation of the posterior hypothalamus with the tip of the cannula enhanced the rate of release of GABA in a frequency-dependent way. Superfusion of the hypothalamus with CSF which contained a high concentration of potassium and a low concentration of sodium increased the rate of release of GABA; this effect was dependent on the presence of calcium ions in the superfusing fluid. Pretreatment of the cats with reserpine reduced the levels of GABA in hypothalamus and rest of brain and the concentration of GABA in the superfusate as well. Stimulation of the locus coeruleus with a bipolar electrode elicited an increased release of GABA in the hypothalamus.

Citing Articles

In vivo release patterns and cardiovascular properties of inhibitory and excitatory amino acids in the hypothalamus.

Singewald N, Guo L, Philippu A Amino Acids. 2013; 8(1):47-58.

PMID: 24186215 DOI: 10.1007/BF00806543.

Release of endogenous GABA in the posterior hypothalamus of the conscious rat; effects of drugs and experimentally induced blood pressure changes.

Singewald N, Guo L, Philippu A Naunyn Schmiedebergs Arch Pharmacol. 1993; 347(4):402-6.

PMID: 8510767 DOI: 10.1007/BF00165390.

In vivo release of endogenous catecholamines, histamine and GABA in the hypothalamus of Wistar Kyoto and spontaneously hypertensive rats.

Tuomisto L, Yamatodani A, Dietl H, Waldmann U, Philippu A Naunyn Schmiedebergs Arch Pharmacol. 1983; 323(3):183-7.

PMID: 6621713 DOI: 10.1007/BF00497660.

Release of endogenous histamine in the hypothalamus of anaesthetized cats and conscious, freely moving rabbits.

Philippu A, Hanesch U, Hagen R, Robinson R Naunyn Schmiedebergs Arch Pharmacol. 1982; 321(4):282-6.

PMID: 6188063 DOI: 10.1007/BF00498514.

Suppression of preoptic GABA release caused by push-pull-perfusion with sodium valproate.

Wolf R, Tscherne U, Emrich H Naunyn Schmiedebergs Arch Pharmacol. 1988; 338(6):658-63.

PMID: 3149723 DOI: 10.1007/BF00165631.

References

Enna S, Snyder S . A simple, sensitive and specific radioreceptor assay for endogenous GABA in brain tissue. J Neurochem. 1976; 26(1):221-4. DOI: 10.1111/j.1471-4159.1976.tb04465.x. View

Obata K, Takeda K . Release of gamma-aminobutyric acid into the fourth ventricle induced by stimulation of the cat's cerebellum. J Neurochem. 1969; 16(7):1043-7. DOI: 10.1111/j.1471-4159.1969.tb05948.x. View

van der Heyden J, Venema K, Korf J . In vivo release of endogenous GABA from rat substantia nigra measured by a novel method. J Neurochem. 1979; 32(2):469-76. View

Biswas B, Carlsson A . The effect of intracerebroventricularly administered GABA on brain monoamine metabolism. Naunyn Schmiedebergs Arch Pharmacol. 1977; 299(1):41-6. DOI: 10.1007/BF00508635. View

Yessaian N, Armenian A, BUNIATIAN H . Effect of gamma-aminobutyric acid on brain serotonin and catecholamines. J Neurochem. 1969; 16(10):1425-33. DOI: 10.1111/j.1471-4159.1969.tb09894.x. View

Chan P, Wong Y, Fishman R . Hyperosmolality-induced GABA release from rat brain slices: studies of calcium dependency and sources of release. J Neurochem. 1978; 30(6):1363-8. DOI: 10.1111/j.1471-4159.1978.tb10467.x. View

Balzer H, HOLTZ P, Palm D . [Studies on the biochemical principles of the convulsive effect of hydrazine]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1960; 239:520-52. View

Sellstrom A, Hamberger A . Potassium-stimulated gamma-aminobutyric acid release from neurons and glia. Brain Res. 1977; 119(1):189-98. DOI: 10.1016/0006-8993(77)90099-3. View

Potashner S . The spontaneous and electrically evoked release, from slices of guinea-pig cerebral cortex, of endogenous amino acids labelled via metabolism of D-[U-14C]glucose. J Neurochem. 1978; 31(1):177-86. DOI: 10.1111/j.1471-4159.1978.tb12446.x. View

10.

Iversen L, Mitchell J, Srinivasan V . The release of gamma-aminobutyric acid during inhibition in the cat visual cortex. J Physiol. 1971; 212(2):519-34. PMC: 1395671. DOI: 10.1113/jphysiol.1971.sp009339. View

11.

Philippu A, Dietl H, Sinha J . In vivo release of endogenous catecholamines in the hypothalamus. Naunyn Schmiedebergs Arch Pharmacol. 1979; 308(2):137-42. DOI: 10.1007/BF00499055. View

12.

Minchin M, Iversen L . Release of (3H)gamma-aminobutyric acid from glial cells in rat dorsal root ganglia. J Neurochem. 1974; 23(3):533-40. DOI: 10.1111/j.1471-4159.1974.tb06056.x. View

13.

Cattabeni F, Maggi A, Monduzzi M, De Angelis L, Racagni G . GABA: circadian fluctuations in rat hypothalamus. J Neurochem. 1978; 31(2):565-7. DOI: 10.1111/j.1471-4159.1978.tb02676.x. View

14.

Philippu A, Przuntek H, Roensberg W . Superfusion of the hypothalamus with gamma-aminobutyric acid. Effect on release of noradrenaline and blood pressure. Naunyn Schmiedebergs Arch Pharmacol. 1973; 276(2):103-18. DOI: 10.1007/BF00501183. View

15.

Enna S, Kuhar M, Snyder S . Regional distribution of postsynaptic receptor binding for gamma-aminobutyric acid (GABA) in monkey brain. Brain Res. 1975; 93(1):168-74. DOI: 10.1016/0006-8993(75)90297-8. View

16.

Tappaz M, Brownstein M . Origin of glutamate-decarboxylase (GAD)-containing cells in discrete hypothalamic nuclei. Brain Res. 1977; 132(1):95-106. DOI: 10.1016/0006-8993(77)90708-9. View