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The -amylase of the Beetle Callosobruchus Chinensis. Properties

Overview
Journal Biochem J
Specialty Biochemistry
Date 1971 Jan 1
PMID 5000646
Citations 4
Authors
H Podoler
S W Applebaum
Affiliations
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Abstract

C. chinensis larval amylase is activated by Ca(2+) and inhibited by Cl(-) and EDTA (K(i) 6.7x10(-3)m). GSH and 2-mercaptoethanol activate, presumably at different sites, as 2-mercaptoethanol interferes with Ca(2+) activation, whereas GSH enhances it. The inhibition by iodoacetic acid and N-ethylmaleimide (K(i) 1.55x10(-2)m) suggest that free thiol groups are essential for activity. The pH optimum of 5.2-5.4 is moved to 5.6-5.8 by Ca(2+) and 2-mercaptoethanol. The activation energy is 7270 cal/mol, and is not affected by Ca(2+) and 2-mercaptoethanol. K(m) for soluble starch is 2.3mg/ml.

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References
1.
MANNING G, Campbell L . Thermostable alpha-amylase of Bacillus stearothermophilus. I. Crystallization and some general properties. J Biol Chem. 1961; 236:2952-7. View
2.
Dixon M . The determination of enzyme inhibitor constants. Biochem J. 1953; 55(1):170-1. PMC: 1269152. DOI: 10.1042/bj0550170. View
3.
Swain R, Dekker E . Seed germination studies. I. Purification and properties of an alpha-amylase from the cotyledons of germinating peas. Biochim Biophys Acta. 1966; 122(1):75-86. DOI: 10.1016/0926-6593(66)90092-0. View
4.
Podoler H, Applebaum S . The -amylase of the beetle Callosobruchus chinensis. Purification and action pattern. Biochem J. 1971; 121(2):317-20. PMC: 1176572. DOI: 10.1042/bj1210317. View
5.
Applebaum S, Konijn A . THE UTILIZATION OF STARCH BY LARVAE OF THE FLOUR BEETLE, TRIBOLIUM CASTANEUM. J Nutr. 1965; 85(3):275-82. DOI: 10.1093/jn/85.3.275. View