Purification and Thermal Stability of Intact Bacillus Subtilis Flagella

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Jan 1

PMID 4993323

Citations 31

Authors

K Dimmitt

M Simon

Affiliations

Soon will be listed here.

Abstract

Flagella were prepared and purified in a relatively intact form from bacterial lysates. Immunochemical tests showed that over 95% of the protein in the final preparation consisted of flagellar antigen. These flagella are more stable to thermal denaturation than flagella filaments obtained by shearing. Their thermal properties more closely resemble those of flagella in the native state on bacteria. The presence of the hook structure is responsible for this extra stability.

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