Isolation of L-forms of Bacillus Subtilis Which Grow in Liquid Medium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1970 Jun 1

PMID 4988045

Citations 13

Authors

F E Young

P Haywood

M Pollock

Affiliations

Soon will be listed here.

Abstract

L-forms of Bacillus subtilis can be isolated by treatment of the parent strain sequentially with N-methyl-N'-nitro-N-nitrosoguanidine and lysozyme and selection of the surviving protoplasts on semisolid medium containing 2,000 units of penicillin per ml. Some of these clones can be adapted to grow in liquid cultures containing 1.2 m NaCl. This method will aid in the isolation of cell wall mutants which require hypertonic medium for growth.

Citing Articles

Discovery of novel cell wall-active compounds using P ywaC, a sensitive reporter of cell wall stress, in the model gram-positive bacterium Bacillus subtilis.

Czarny T, Perri A, French S, Brown E Antimicrob Agents Chemother. 2014; 58(6):3261-9.

PMID: 24687489 PMC: 4068450. DOI: 10.1128/AAC.02352-14.

Temperature-Sensitive Osmotically Fragile Mutants of Staphylococcus aureus.

Good C, Pattee P J Bacteriol. 1970; 104(3):1401-3.

PMID: 16559123 PMC: 248307. DOI: 10.1128/jb.104.3.1401-1403.1970.

Transformation of a Bacillus subtilis L-form with bacteriophage deoxyribonucleic acid.

White T, Doyle R, Streips U J Bacteriol. 1981; 145(2):878-83.

PMID: 6780533 PMC: 217193. DOI: 10.1128/jb.145.2.878-883.1981.

Quantitation of bacillus subtilis L-form growth parameters in batch culture.

Gilpin R, Patterson S, Knight R J Bacteriol. 1981; 145(1):651-3.

PMID: 6780526 PMC: 217320. DOI: 10.1128/jb.145.1.651-653.1981.

Bacterial growth and division: genes, structures, forces, and clocks.

Mendelson N Microbiol Rev. 1982; 46(3):341-75.

PMID: 6182451 PMC: 281548. DOI: 10.1128/mr.46.3.341-375.1982.

References

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