Effect of Polyamines on the Stability of Brain-cortex Ribosomes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1969 Oct 1

PMID 4981036

Citations 5

Authors

R K Datta

S Sen

J J Ghosh

Affiliations

Soon will be listed here.

Abstract

1. Ribosomes isolated from the cortex tissue of goat brain contain very small amounts of spermidine and spermine. Ribosomes isolated from spermidine-treated slices have a higher spermidine content. 2. The polyamines partially prevent the temperature-dependent breakdown of ribosomes into acid-soluble nucleotides. 3. The ;melting' temperature of ribosomes rises slightly when the ribosomes are heated slowly in the presence of polyamines. 4. The pH-dependent breakdown of ribosomes into protein, RNA and acid-soluble nucleotide is markedly decreased by polyamines present in media in which ribosomes are suspended. 5. The breakdown of ribosomes in the presence of high concentrations of salts and EDTA is partially checked by the concurrent presence of polyamines. 6. Spermidine and spermine make ribosomes less susceptible to enzymic digestion by crystalline trypsin and ribonuclease.

Citing Articles

The chloroplast and cytoplasmic ribosomes of euglena: I. Stability of chloroplast ribosomes prepared by an improved procedure.

Schwartzbach S, Freyssinet G, Schiff J Plant Physiol. 1974; 53(4):533-42.

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Polyamines and amino acid incorporation in vitro into microsomes of rat cerebral cortex.

Giorgi P Biochem J. 1970; 120(3):643-51.

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Mescaline-induced changes of brain-cortex ribosomes. Role of sperimidine in counteracting the destabilizing effect of mescaline of brain-cortex ribosomes.

Datta R, ANTOPOL W, Ghosh J Biochem J. 1971; 125(1):213-9.

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Mescaline-induced changes of brain-cortex ribosomes. Effect of mescaline on the stability of brain-cortex ribosomes.

Datta R, Ghosh J Biochem J. 1970; 117(5):961-8.

PMID: 4318016 PMC: 1179057. DOI: 10.1042/bj1170961.

Polyamines, ribonucleases, and the stability of RNA.

Karpetsky T, Hieter P, Frank J, Levy C Mol Cell Biochem. 1977; 17(2):89-99.

PMID: 333273 DOI: 10.1007/BF01743432.

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