Biological Activity of 5-hydroxymethyluracil and Its Deoxynucleoside in Noninfected and Phage-infected Bacillus Subtilis

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1969 Feb 1

PMID 4975365

Citations 4

Authors

M Nishihara

N Friedman

H Vasken Aposhian

Affiliations

Soon will be listed here.

Abstract

(14)C-hydroxymethyldeoxyuridine (dHMU) is specifically incorporated into the deoxyribonucleic acid (DNA) of bacteriophage SP8. Incorporation experiments demonstrate that the initiation of phage SP8 DNA synthesis occurs between 12.5 to 15 min after infection. Incorporation into host DNA does not occur. (14)C-dHMU can be used as an analytical tool for screening conditionally lethal phage mutants containing hydroxymethyluracil in their DNA to select those that are defective in DNA synthesis under restrictive conditions. The pyrimidine, (14)C-hydroxymethyluracil (HMU), is not incorporated into bacterial or phage DNA. Neither HMU nor dHMU can replace thymine as a growth requirement for Bacillus subtilis 168 Ind(-) Thy(-). HMU does not inhibit the utilization of thymine. Although dHMU inhibits deoxythymidine utilization, the inhibition is not competitive.

Citing Articles

Dominance relationships in mixedly infected Bacillus subtilis.

Palefski S, Hemphill H, Kolenbrander P, WHITELEY H J Virol. 1972; 9(4):594-601.

PMID: 4623223 PMC: 356343. DOI: 10.1128/JVI.9.4.594-601.1972.

Synthesis of the unusual DNA of Bacillus subtilis bacteriophage SP-15.

Neubort S, MARMUR J J Virol. 1973; 12(5):1078-84.

PMID: 4203083 PMC: 356739. DOI: 10.1128/JVI.12.5.1078-1084.1973.

Bacteriophages of Bacillus subtilis.

Hemphill H, WHITELEY H Bacteriol Rev. 1975; 39(3):257-315.

PMID: 809034 PMC: 413919. DOI: 10.1128/br.39.3.257-315.1975.

Biosynthesis of 5-(4'5'-dihydroxypentyl) uracil as a nucleoside triphosphate in bacteriophage SP15-infected Bacillus subtilis.

Walker M, Mandel M J Virol. 1978; 25(2):500-9.

PMID: 146749 PMC: 353962. DOI: 10.1128/JVI.25.2.500-509.1978.

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