Effect of PH on the Immunogenicity of Mycoplasma Pneumoniae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1969 Feb 1

PMID 4975150

Citations 21

Authors

J D Pollack

N L Somerson

L B Senterfit

Affiliations

Soon will be listed here.

Abstract

Mycoplasma pneumoniae harvested from media which had become acid lost the ability both to induce formation of tetrazolium reduction inhibition antibody and to act as antigens in immunodiffusion against human convalescent-phase sera. Incorporation of N-tris(hydroxymethyl)-2-aminoethane sulfonic acid and N-2-hydroxyethylpiperazine-N'-2-ethane sulfonic acid buffers into a new medium containing PPLO Serum Fraction instead of horse serum delayed the pH decline. Tris(hydroxymethyl)aminomethane, triethanolamine, and 3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid buffers inhibited growth. Mycoplasmas obtained from buffered cultures retained antigenicity as measured by immunodiffusion and could stimulate tetrazolium reduction inhibition antibody formation in animals.

Citing Articles

Model-driven design allows growth of Mycoplasma pneumoniae on serum-free media.

Gaspari E, Malachowski A, Garcia-Morales L, Burgos R, Serrano L, Martins Dos Santos V NPJ Syst Biol Appl. 2020; 6(1):33.

PMID: 33097709 PMC: 7584665. DOI: 10.1038/s41540-020-00153-7.

Immunogenicity of Mycoplasma pneumoniae.

KENNY G Infect Immun. 1971; 3(4):510-5.

PMID: 16558007 PMC: 416186. DOI: 10.1128/iai.3.4.510-515.1971.

Isolation, Characterization, and Immunogenicity of Mycoplasma pneumoniae Membranes.

Pollack J, Somerson N, Senterfit L Infect Immun. 1970; 2(3):326-39.

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Effect of Animal Passage on Arthritogenic and Biological Properties of Mycoplasma arthritidis.

Golightly-Rowland L, Cole B, WARD J, Wiley B Infect Immun. 1970; 1(6):538-45.

PMID: 16557773 PMC: 415939. DOI: 10.1128/iai.1.6.538-545.1970.

Respiration-associated components of Mollicutes.

Pollack J, Merola A, Platz M, Booth Jr R J Bacteriol. 1981; 146(3):907-13.

PMID: 7240087 PMC: 216943. DOI: 10.1128/jb.146.3.907-913.1981.

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