Lysis of Staphylococcus Aureus Cell Walls by a Soluble Staphylococcal Enzyme

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1968 Jan 1

PMID 4965982

Citations 17

Authors

E Huff

C S SILVERMAN

Affiliations

Soon will be listed here.

Abstract

Enzyme preparations of Staphylococcus aureus were examined for their ability to solubilize (32)P-labeled cell walls of the parent organism. Enzymatic activity was observed in the growth medium, in soluble fractions, and associated with native cell walls. Enzyme associated with isolated cell walls could be inactivated with formaldehyde without reducing the susceptibility of the walls to the action of added enzyme. When cells are frozen and thawed, 50 to 75% of the intracellular enzyme is released along with 2% of the intracellular protein. This freeze-thaw extracted enzyme has little, if any, activity on intact S. aureus cells. It appears that the enzyme resides near the cell wall and acts on the cell-wall inner surface.

Citing Articles

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Extracellular cell wall lytic enzyme from Staphylococcus aureus: purification and partial characterization.

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