Article: Cellular immunity in vitro: migration inhibition and phagocytosis

The activity of peritoneal exudate cells from candidin-sensitive and normal guinea pigs with and without antigen was studied in vitro with the aid of time-lapse, phase-contrast cinemicrography. Macrophages from normal animals migrate well on an agar medium and readily phagocytose Merthiolate-killed Candida albicans cells. A reduction in the migration of peritoneal macrophages from guinea pigs sensitized to C. albicans during the first 24 hr after exposure to antigen was accompanied by a decrease in phagocytosis of C. albicans cells. The macrophages were viable but comparatively immotile. Since the sensitized macrophages came from resistant donors, it is possible that the initial stage of cellular immunity to C. albicans is associated with a reduced activity of the phagocytic macrophages, apparently to limit spread of the pathogen from the infected area.

Citing Articles

In vitro phagocytosis of Candida albicans by peritoneal mouse macrophages.

EVRON R Infect Immun. 1980; 28(3):963-71.

PMID: 6995329 PMC: 551045. DOI: 10.1128/iai.28.3.963-971.1980.

The development of the human lymph node.

Markgraf R, von Gaudecker B, Muller-Hermelink H Cell Tissue Res. 1982; 225(2):387-413.

PMID: 6980711 DOI: 10.1007/BF00214691.

Specific macrophage immunity to vaccinia virus: macrophage-virus interaction.

Avila F, Schultz R, Tompkins W Infect Immun. 1972; 6(1):9-16.

PMID: 4564156 PMC: 422482. DOI: 10.1128/iai.6.1.9-16.1972.

On the interdigitating cells in the thymus-dependent area of the rat spleen: a relation between the mononuclear phagocyte system and T-lymphocytes.

Veerman A Cell Tissue Res. 1974; 148(2):247-57.

PMID: 4546010 DOI: 10.1007/BF00224586.

Pathological observations in experimental candida infection of sensitized guinea pigs.

ISODA M Mycopathologia. 1985; 91(3):187-92.

PMID: 4058565 DOI: 10.1007/BF00446299.

References

1.

GRANGER G, Shacks S, Williams T, KOLB W . Lymphocyte in vitro cytotoxicity: specific release of lymphotoxin-like materials from tuberculin-sensitive lymphoid cells. Nature. 1969; 221(5186):1155-7. DOI: 10.1038/2211155a0. View

2.

SALVIN S, Nishio J, Gribik M . Lymphoid cells in delayed hypersensitivity. 1. In vitro vs. in vivo responses. Cell Immunol. 1970; 1(1):62-77. DOI: 10.1016/0008-8749(70)90061-4. View

3.

SALVIN S, Nishio J . Communications. "In vitro" cell reactions in delayed hypersensitivity. J Immunol. 1969; 103(1):138-41. View

4.

Nathan C, Karnovsky M, David J . Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971; 133(6):1356-76. PMC: 2138934. DOI: 10.1084/jem.133.6.1356. View

5.

FAUVE R, Dekaris D . Macrophage spreading: inhibition in delayed hypersensitivity. Science. 1968; 160(3829):795-6. DOI: 10.1126/science.160.3829.795. View

6.

SALVIN S, Sell S, Nishio J . Activity in vitro of lymphocytes and macrophages in delayed hypersensitivity. J Immunol. 1971; 107(3):655-62. View

7.

NORTH R . The uptake of particulate antigens. J Reticuloendothel Soc. 1968; 5(3):203-29. View

8.

SALVIN S, Cheng S . Lymphoid Cells in Delayed Hypersensitivity II. In Vitro Phagocytosis and Cellular Immunity. Infect Immun. 1971; 3(4):548-52. PMC: 416194. DOI: 10.1128/iai.3.4.548-552.1971. View

9.

Mackaness G . The monocyte in cellular immunity. Semin Hematol. 1970; 7(2):172-84. View

10.

Bennett B, Bloom B . Reactions in vivo and in vitro produced by a soluble substance associated with delayed-type hypersensitivity. Proc Natl Acad Sci U S A. 1968; 59(3):756-62. PMC: 224740. DOI: 10.1073/pnas.59.3.756. View

11.

Ward P, Remold H, David J . The production by antigen-stimulated lymphocytes of a leukotactic factor distinct from migration inhibitory factor. Cell Immunol. 1970; 1(2):162-74. DOI: 10.1016/0008-8749(70)90003-1. View

12.

Waksman B, MATOLTSY M . The effect of tuberculin on peritoneal exudate cells of sensitized guinea pigs in surviving cell culture. J Immunol. 1958; 81(3):220-34. View

13.

George M, Vaughan J . In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962; 111:514-21. DOI: 10.3181/00379727-111-27841. View

Cellular Immunity in Vitro: Migration Inhibition and Phagocytosis