Immunity in Experimental Salmonellosis. II. Basis for the Avirulence and Protective Capacity of Gal E Mutants of Salmonella Typhimurium

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1971 Dec 1

PMID 4949507

Citations 36

Authors

R Germanier

E Furer

Affiliations

Soon will be listed here.

Abstract

Salmonella typhimurium strains which are deficient in uridine diphosphate (UDP)-galactose-4-epimerase (gal E mutants) owe their outstanding protective capacity when used as live vaccine to the fact that when galactose is supplied exogenously, such as occurs in vivo, smooth cell wall lipopolysaccharides are synthesized. The mutants lose most of their protective capacity when this phenotypic curing is prevented by a second mutation of the kind found in strains LT(2)M(1)A (deficient in galactokinase) or E(32) (deficient in UDP-galactose-lipopolysaccharide transferase). Despite such phenotypic reversion, the gal E mutants are rendered avirulent as a result of galactose-induced bacteriolysis. Secondary mutants have been isolated which differ from each other with respect to the extent of galactose-induced lysis. The differences in galactose sensitivity are attributable to different activities of the other Leoloir pathway enzymes, namely, galactokinase and galactose-1-phosphate-uridyl transferase. The influence of these enzymes on lipopolysaccharide composition and galactose sensitivity and thus on virulence and immunogenicity of gal E mutants has been studied.

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References

Krishnapillai V, MacPhee D, Stocker B . Properties of a Salmonella typhimurium mutant with an incomplete deficiency of uridinediphosphogalactose-4-epimerase. J Bacteriol. 1971; 107(1):155-61. PMC: 246899. DOI: 10.1128/jb.107.1.155-161.1971. View

Osborn M . STUDIES ON THE GRAM-NEGATIVE CELL WALL. I. EVIDENCE FOR THE ROLE OF 2-KETO- 3-DEOXYOCTONATE IN THE LIPOPOLYSACCHARIDE OF SALMONELLA TYPHIMURIUM. Proc Natl Acad Sci U S A. 1963; 50:499-506. PMC: 221208. DOI: 10.1073/pnas.50.3.499. View

Friedberg D, Shilo M . Role of cell wall structure of salmonella in the interaction with phagocytes. Infect Immun. 1970; 2(3):279-85. PMC: 416002. DOI: 10.1128/iai.2.3.279-285.1970. View

Ushiba D . Two types of immunity in experimental typhoid; "cellular immunity" and "humoral immunity". Keio J Med. 1965; 14(2):45-61. DOI: 10.2302/kjm.14.45. View

GEMSKI Jr P, Stocker B . Transduction by bacteriophage P22 in nonsmooth mutants of Salmonella typhimurium. J Bacteriol. 1967; 93(5):1588-97. PMC: 276654. DOI: 10.1128/jb.93.5.1588-1597.1967. View

Germanier R . Immunity in Experimental Salmonellosis I. Protection Induced by Rough Mutants of Salmonella typhimurium. Infect Immun. 1970; 2(3):309-15. PMC: 416007. DOI: 10.1128/iai.2.3.309-315.1970. View

Kenny K, Herzberg M . Antibody response and protection induced by immunization with smooth and rough strains in experimental salmonellosis. J Bacteriol. 1968; 95(2):406-17. PMC: 252033. DOI: 10.1128/jb.95.2.406-417.1968. View

Osborn M, Rosen S, Rothfield L, HORECKER B . Biosynthesis of bacterial lipopolysaccharide. I. Enzymatic incorporation of galactose in a mutant strain of Salmonella. Proc Natl Acad Sci U S A. 1962; 48:1831-8. PMC: 221047. DOI: 10.1073/pnas.48.10.1831. View

KENT J, Osborn M . Further studies on enzymatic synthesis of O-antigen in Salmonella typhimurium. Biochemistry. 1968; 7(12):4409-19. DOI: 10.1021/bi00852a037. View

10.

Osborn M . Structure and biosynthesis of the bacterial cell wall. Annu Rev Biochem. 1969; 38:501-38. DOI: 10.1146/annurev.bi.38.070169.002441. View

11.

Osborn M, Rosen S, Rothfield L, ZELEZNICK L, HORECKER B . LIPOPOLYSACCHARIDE OF THE GRAM-NEGATIVE CELL WALL. Science. 1964; 145(3634):783-9. DOI: 10.1126/science.145.3634.783. View

12.

Hashimoto H, Honda T, Kawakami M, Mitsuhashi S . Studies on the experimental salmonellosis. VI. Longlasting immunity of mouse immunized with live vaccine of Salmonella enteritidis. Jpn J Exp Med. 1961; 31:187-90. View

13.

Lindberg A, Hellerqvist C . Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium. J Bacteriol. 1971; 105(1):57-64. PMC: 248321. DOI: 10.1128/jb.105.1.57-64.1971. View

14.

Nakano M, Saito K . Antibody formation in mice infected with Salmonella typhimurium by primary immunization with sheep erythrocytes or bacterial cells. Jpn J Microbiol. 1970; 14(1):73-81. DOI: 10.1111/j.1348-0421.1970.tb00493.x. View

15.

Fukasawa T, Nikaido H . Galactose-sensitive mutants of Salmonella. II. Bacteriolysis induced by galactose. Biochim Biophys Acta. 1961; 48:470-83. DOI: 10.1016/0006-3002(61)90045-2. View

16.

Nikaido H . Biosynthesis of cell wall lipopolysaccharide in gram-negative enteric bacteria. Adv Enzymol Relat Areas Mol Biol. 1968; 31:77-124. DOI: 10.1002/9780470122761.ch3. View

17.

Fukasawa T, Nikaido H . Formation of protoplasts' in mutant strains of Salmonella induced by galactose. Nature. 1959; 183(4668):1131-2. DOI: 10.1038/1831131a0. View

18.

Lennox E . Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955; 1(2):190-206. DOI: 10.1016/0042-6822(55)90016-7. View

19.

Buttin G . [REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. I. THE INDUCED BIOSYNTHESIS OF GALACTOKINASE AND THE SIMULTANEOUS INDUCTION OF THE ENZYMATIC SEQUENCE]. J Mol Biol. 1963; 7:164-82. DOI: 10.1016/s0022-2836(63)80044-3. View

20.

Fukasawa T, Nikaido H . Galactose-sensitive mutants of Salmonella. Nature. 1959; 184(Suppl 15):1168-9. DOI: 10.1038/1841168a0. View