Use of Yeast Populations Fractionated by Zonal Centrifugation to Study the Cell Cycle

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Dec 1

PMID 4945182

Citations 25

Authors

J Sebastian

B L Carter

H O HALVORSON

Affiliations

Soon will be listed here.

Abstract

Zonal centrifugation in a sucrose density gradient was used to separate yeast cells primarily by size and thus by age in the cell cycle. This approach provides an alternative to synchronous growth for examining the properties of cells at different stages in the cell cycle.

Citing Articles

Identification of proteins whose synthesis is modulated during the cell cycle of Saccharomyces cerevisiae.

Lorincz A, Miller M, Xuong N, Geiduschek E Mol Cell Biol. 1982; 2(12):1532-49.

PMID: 14582195 PMC: 369962. DOI: 10.1128/mcb.2.12.1532-1549.1982.

Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.

Ludwig 2nd J, Foy J, Elliott S, McLaughlin C Mol Cell Biol. 1982; 2(2):117-26.

PMID: 7050667 PMC: 369764. DOI: 10.1128/mcb.2.2.117-126.1982.

Small-sized mutants of Saccharomyces cerevisiae.

Carter B, Sudbery P Genetics. 1980; 96(3):561-6.

PMID: 7021310 PMC: 1214361. DOI: 10.1093/genetics/96.3.561.

Changes in the activity and properties of trehalase during early germination of yeast ascospores: correlation with trehalose breakdown as studied by in vivo 13C NMR.

Thevelein J, den Hollander J, Shulman R Proc Natl Acad Sci U S A. 1982; 79(11):3503-7.

PMID: 6954495 PMC: 346449. DOI: 10.1073/pnas.79.11.3503.

Saccharomyces cerevisiae cell cycle.

Hartwell L Bacteriol Rev. 1974; 38(2):164-98.

PMID: 4599449 PMC: 413849. DOI: 10.1128/br.38.2.164-198.1974.

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