Control of Single Ribosome Formation by an Initiation Factor for Protein Synthesis

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 Oct 1

PMID 4944628

Citations 4

Authors

R KAEMPFER

Affiliations

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Abstract

30 and 50S ribosomal subunits, released from polysomes upon polypeptide chain termination, possess a high affinity for each other and readily form single ribosomes. Highly purified initiation factor F3(B), acting stoichiometrically, prevents the formation of single ribosomes without promoting their dissociation. These findings are interpreted in terms of a ribosome cycle in which a limiting amount of factor F3(B) controls the number of ribosomes active in protein synthesis, in response to metabolic changes in the cell, by regulating the flow of ribosomal subunits into polysomes or into a sidetrack pool of synthetically inactive single ribosomes. The reported apparent ribosome dissociation activity of F3(B) is explained.

Citing Articles

Studies on the interaction between ribosomes and 14 CH 3 -F 3 initation factor.

Pon C, Friedman S, GUALERZI C Mol Gen Genet. 1972; 116(2):192-8.

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Factor-promoted dissociation of free ribosomes in a rabbit reticulocyte lysate system: inhibition and requirement for an energy source.

Mizuno S, Rabinovitz M Proc Natl Acad Sci U S A. 1973; 70(3):787-91.

PMID: 4514991 PMC: 433359. DOI: 10.1073/pnas.70.3.787.

Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA.

KAEMPFER R, Kaufman J Proc Natl Acad Sci U S A. 1972; 69(11):3317-21.

PMID: 4508325 PMC: 389762. DOI: 10.1073/pnas.69.11.3317.

Neuronal perikarya with dispersed, single ribosomes in the visual cortex of Macaca mulatta.

PALAY S, Billings-Gagliardi S, Chan-Palay V J Cell Biol. 1974; 63(3):1074-89.

PMID: 4436380 PMC: 2109373. DOI: 10.1083/jcb.63.3.1074.

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