An Escherichia Coli Mutant with Increased Messenger Ribonuclease Activity

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 Dec 1

PMID 4943555

Citations 13

Authors

E T Lennette

L Gorelic

D Apirion

Affiliations

Soon will be listed here.

Abstract

A temperature-sensitive mutant strain of Escherichia coli exhibits a remarkable increase in RNase activity when grown at its nonpermissive temperature. During growth at the nonpermissive temperature, there is an increase in the extent of breakdown of pulse-labeled RNA and a decrease in the functional lifetime of the mRNA for the lac operon. T7 RNA, which is usually stable in E. coli, is also stable in this strain at the nonpermissive temperature. It is possible that the RNase measured is part of the normal mechanism of mRNA degradation in the cell. A mechanism for mRNA degradation that requires the combined action of endonuclease(s) and 3' to 5' exonuclease(s) is proposed.

Citing Articles

Unaltered stability of newly synthesized RNA in strains of Escherichia coli missing a ribonuclease specific for double-stranded RNA.

Apirion D, Watson N Mol Gen Genet. 1975; 136(4):317-26.

PMID: 16094999 DOI: 10.1007/BF00341716.

Amplification of ribonuclease II (rnb) activity in Escherichia coli K-12.

Donovan W, Kushner S Nucleic Acids Res. 1983; 11(2):265-75.

PMID: 6338477 PMC: 325713. DOI: 10.1093/nar/11.2.265.

Decay of RNA in RNA processing mutants of Escherichia coli.

Apirion D, Gitelman D Mol Gen Genet. 1980; 177(2):339-43.

PMID: 6154228 DOI: 10.1007/BF00267448.

RNA precursor molecules and ribonucleases in E. coli.

Altman S, Robertson H Mol Cell Biochem. 1973; 1(1):83-93.

PMID: 4610352 DOI: 10.1007/BF01659941.

Analysis of an Escherichia coli strain carrying physiologically compensating mutations one of which causes an altered ribonuclease 3.

Apirion D, Watson N Mol Gen Genet. 1974; 132(2):89-104.

PMID: 4609394 DOI: 10.1007/BF00272175.

References

GESTELAND R . Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966; 16(1):67-84. DOI: 10.1016/s0022-2836(66)80263-2. View

Kepes A . KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963; 76:293-309. View

Nossal N, Singer M . The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II. J Biol Chem. 1968; 243(5):913-22. View

Elson D . A correlation between ribonuclease II and the in vivo inactivation of messenger RNA in E. coli. Biochem Biophys Res Commun. 1968; 33(3):412-7. DOI: 10.1016/0006-291x(68)90587-1. View

Di Mauro E, Synder L, Marino P, Lamberti A, Coppo A, Tocchini-Valentini G . Rifampicin sensitivity of the components of DNA-dependent RNA polymerase. Nature. 1969; 222(5193):533-7. DOI: 10.1038/222533a0. View

Morikawa N, Imamoto F . Degradation of tryptophan messenger. On the degradation of messenger RNA for the tryptophan operon in Escherichia coli. Nature. 1969; 223(5201):37-40. DOI: 10.1038/223037a0. View

Morse D, Mosteller R, BAKER R, Yanofsky C . Direction of in vivo degradation of tryptophan messenger RNA--a correction. Nature. 1969; 223(5201):40-3. DOI: 10.1038/223040a0. View

Phillips S, Schlessinger D, Apirion D . Mutants in Escherichia coli ribosomes: a new selection. Proc Natl Acad Sci U S A. 1969; 62(3):772-7. PMC: 223665. DOI: 10.1073/pnas.62.3.772. View

Castles J, Singer M . Degradation of polyuridylic acid by ribonuclease II: protection by ribosomes. J Mol Biol. 1969; 40(1):1-17. DOI: 10.1016/0022-2836(69)90292-7. View

10.

Morse D, Primakoff P . Relief of polarity in E. coli by "suA". Nature. 1970; 226(5240):28-31. DOI: 10.1038/226028a0. View

11.

Apirion D, Phillips S, Schlessinger D . Approaches to the genetics of Escherichia coli ribosomes. Cold Spring Harb Symp Quant Biol. 1969; 34:117-28. DOI: 10.1101/sqb.1969.034.01.018. View

12.

Phillips S, Schlessinger D, Apirion D . Temperature-dependent suppression of UGA and UAA codons in a temperature-sensitive mutant of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1969; 34:499-503. DOI: 10.1101/sqb.1969.034.01.056. View

13.

Morse D, Mosteller R, Yanofsky C . Dynamics of synthesis, translation, and degradation of trp operon messenger RNA in E. coli. Cold Spring Harb Symp Quant Biol. 1969; 34:725-40. DOI: 10.1101/sqb.1969.034.01.082. View

14.

Taylor A . Current linkage map of Escherichia coli. Bacteriol Rev. 1970; 34(2):155-75. PMC: 408314. DOI: 10.1128/br.34.2.155-175.1970. View

15.

Chamberlin M, McGrath J, Waskell L . New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970; 228(5268):227-31. DOI: 10.1038/228227a0. View

16.

Summers W . The process of infection with coliphage T7. IV. Stability of RNA in bacteriophage-infected cells. J Mol Biol. 1970; 51(3):671-8. DOI: 10.1016/0022-2836(70)90015-x. View

17.

Schwartz T, Craig E, Kennell D . Inactivation and degradation of messenger ribnucleic acid from the lactose operon of Escherichia coli. J Mol Biol. 1970; 54(2):299-311. DOI: 10.1016/0022-2836(70)90431-6. View

18.

Gorelic L, Apirion D . Inhibition of RNase II by DNA. Biochem Biophys Res Commun. 1971; 44(5):1184-91. DOI: 10.1016/s0006-291x(71)80211-5. View

19.

Hartmann G, Honikel K, KNUSEL F, Nuesch J . The specific inhibition of the DNA-directed RNA synthesis by rifamycin. Biochim Biophys Acta. 1967; 145(3):843-4. DOI: 10.1016/0005-2787(67)90147-5. View