Article: Inhibition of arginyl-transfer ribonucleic acid synthetase activity of Escherichia coli by arginine biosynthetic precursors

The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next, and citrulline is least effective. The implications of these results are discussed in relation to arginyl-tRNA synthetase activity and the level of the arginine biosynthetic enzymes during conditions of restricted and unrestricted supply of arginine to cells.

Citing Articles

Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa.

Economidis I, Wagner R Wilehm Roux Arch Dev Biol. 2017; 189(3):171-180.

PMID: 28305172 DOI: 10.1007/BF00868675.

Control of arginine biosynthesis in Escherichia coli: inhibition of arginyl-transfer ribonucleic acid synthetase activity.

Williams A, Yem D, McGinnis E, Williams L J Bacteriol. 1973; 115(1):228-34.

PMID: 4577743 PMC: 246234. DOI: 10.1128/jb.115.1.228-234.1973.

Control of arginine biosynthesis in Escherichia coli: role of arginyl-transfer ribonucleic acid synthetase in repression.

Williams L J Bacteriol. 1973; 113(3):1419-32.

PMID: 4570785 PMC: 251713. DOI: 10.1128/jb.113.3.1419-1432.1973.

References

1.

SILBERT D, Fink G, Ames B . Histidine regulatory mutants in Salmonella typhimurium 3. A class of regulatory mutants deficient in tRNA for histidine. J Mol Biol. 1966; 22(2):335-47. DOI: 10.1016/0022-2836(66)90136-7. View

2.

NAZARIO M . The accumulation of argininosuccinate in Neurospora crassa. II. Inhibition of arginyl-tRNA synthesis by argininosuccinate. Biochim Biophys Acta. 1967; 145(1):146-52. DOI: 10.1016/0005-2787(67)90663-6. View

3.

Chrispeels M, Boyd R, Williams L, Neidhardt F . Modification of valyl tRNA synthetase by bacteriophage in Escherichia coli. J Mol Biol. 1968; 31(3):463-75. DOI: 10.1016/0022-2836(68)90421-x. View

4.

Williams L, Neidhardt F . Synthesis and inactivation of aminoacyl-transfer RNA synthetases during growth of Escherichia coli. J Mol Biol. 1969; 43(3):529-50. DOI: 10.1016/0022-2836(69)90357-x. View

5.

Hirshfield I, Horn P, Hopwood D, Maas W, DeDeken R . Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants. J Mol Biol. 1968; 35(1):83-93. DOI: 10.1016/s0022-2836(68)80038-5. View

6.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

7.

Novick R, Maas W . Control by endogenously synthesized arginine of the formation of ornithine transcarbamylase in Escherichia coli. J Bacteriol. 1961; 81:236-40. PMC: 278992. DOI: 10.1128/jb.81.2.236-240.1961. View

8.

GORINI L, GUNDERSEN W, Burger M . Genetics of regulation of enzyme synthesis in the arginine biosynthetic pathway of Escherichia coli. Cold Spring Harb Symp Quant Biol. 1961; 26:173-82. DOI: 10.1101/sqb.1961.026.01.022. View

9.

Schlesinger S, MAGASANIK B . EFFECT OF ALPHA-METHYLHISTIDINE ON THE CONTROL OF HISTIDINE SYNTHESIS. J Mol Biol. 1964; 9:670-82. DOI: 10.1016/s0022-2836(64)80174-1. View

10.

Lennox E . Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955; 1(2):190-206. DOI: 10.1016/0042-6822(55)90016-7. View

11.

Sercarz E, GORINI L . DIFFERENT CONTRIBUTION OF EXOGENOUS AND ENDOGENOUS ARGININE TO REPRESSOR FORMATION. J Mol Biol. 1964; 8:254-62. DOI: 10.1016/s0022-2836(64)80135-2. View

Inhibition of Arginyl-transfer Ribonucleic Acid Synthetase Activity of Escherichia Coli by Arginine Biosynthetic Precursors