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Growth Rate of Escherichia Coli at Elevated Temperatures: Limitation by Methionine

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1971 Aug 1
PMID 4939758
Citations 52
Authors
E Z Ron
B D Davis
Affiliations
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Abstract

When Escherichia coli growing in minimal medium is shifted from 37 C to any temperature in the range 40 to 45 C, the growth rate immediately assumes a new, lower value, characteristic of that temperature. The decrease is shown to be due, in several strains, to decreased activity of the first enzyme of the methionine pathway, homoserine trans-succinylase, which thus appears to be more heat-sensitive than any other essential enzyme in the cell. This sensitivity does not involve progressive denaturation of the enzyme; rather, the response to a shift of temperature, in either direction, is immediate and reversible.

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