New Method for Extraction of Ribonucleic Acid and Polyribosomes from Schizosaccharomyces Pombe

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Sep 1

PMID 4937781

Citations 5

Authors

H C Birnboim

Affiliations

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Abstract

A new method for extracting ribosomal ribonucleic acid (RNA) and polyribosomes from Schizosaccharomyces pombe, a fission yeast, has been developed. Cells grown in the presence of 2-deoxyglucose were found to be more sensitive to the action of Glusulase; a short period of treatment (10 to 20 min) at 0 C was sufficient to convert the cells to osmotically sensitive structures. From these, ribosomal RNA and polyribosomes were readily prepared.

Citing Articles

Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombe.

Birnboim H, Nasim A Mol Gen Genet. 1975; 136(1):1-8.

PMID: 16094962 DOI: 10.1007/BF00275444.

Effect of 2-deoxy-D-glucose on growth and cell walls of Schizosaccharomyces pombe 972h.

Poole R, Lloyd D Arch Mikrobiol. 1973; 88(4):257-72.

PMID: 4684596 DOI: 10.1007/BF00409939.

Detection of pyrimidine dimers in hydrolysates of yeast DNA by high voltage paper electrophoresis.

Birnboim H, Nassim A Mol Gen Genet. 1974; 130(4):291-6.

PMID: 4604306 DOI: 10.1007/BF00333867.

Recessive super-suppression in yeast.

Smirnov V, Kreier V, Lizlova L, Andrianova V, Inge-Vechtomov S Mol Gen Genet. 1974; 129(2):105-21.

PMID: 4598794 DOI: 10.1007/BF00268625.

Morphogenic effect of 2-deoxy-D-arabino-hexose on Rhodosporidium toruloides.

Sipiczki M, Farkas V Folia Microbiol (Praha). 1979; 24(5):389-95.

PMID: 527913 DOI: 10.1007/BF02927121.

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