Auxotroph Accumulation in Deoxyribonucleic Acid Polymeraseless Strains of Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Jun 1

PMID 4934065

Citations 12

Authors

C M Berg

Affiliations

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Abstract

Spontaneous auxotrophs are found with high frequency in several strains of Escherichia coli K-12 deficient in Kornberg deoxyribonucleic acid polymerase. These include amino acid-, vitamin-, purine-, and pyrimidine-requiring strains. Although this was suggestive evidence that these strains might be mutators, reconstruction experiments demonstrate that auxotrophs possess a selective advantage over prototrophs in the same culture. Thus, despite the high frequency of auxotrophs in polymerase-deficient strains, it is not yet clear whether they have elevated mutation rates.

Citing Articles

Powerful mutator activity of the polA1 mutation within the histidine region of Escherichia coli K-12.

Savic D, Romac S J Bacteriol. 1982; 149(3):955-60.

PMID: 7037745 PMC: 216483. DOI: 10.1128/jb.149.3.955-960.1982.

Trimethoprim-induced DNA polymerase I deficiency in Escherichia coli K-12.

Mukhopadhyay P, Syrowik U, Palchaudhuri S J Bacteriol. 1983; 154(3):1098-103.

PMID: 6343343 PMC: 217579. DOI: 10.1128/jb.154.3.1098-1103.1983.

Thymineless death in polA+ and polA- strains of Escherichia coli.

Berg C, ONeill J J Bacteriol. 1973; 115(2):707-8.

PMID: 4579878 PMC: 246303. DOI: 10.1128/jb.115.2.707-708.1973.

Linkage map of Escherichia coli strain K-12.

Taylor A, Trotter C Bacteriol Rev. 1972; 36(4):504-24.

PMID: 4568762 PMC: 408330. DOI: 10.1128/br.36.4.504-524.1972.

Mutator genes in different species.

Mohn G, Wurgler F Humangenetik. 1972; 16(1):49-58.

PMID: 4567210 DOI: 10.1007/BF00393985.

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