Cytological Studies of Deoxyribonucleic Acid Replication in Escherichia Coli 15T-: Replication at Slow Growth Rates and After a Shift-up into Rich Medium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1970 Oct 1

PMID 4919753

Citations 27

Authors

N C Chai

K G Lark

Affiliations

Soon will be listed here.

Abstract

We examined the gross nuclear morphology of Escherichia coli 15T(-) grown in different media with doubling times ranging from 22 to 270 min. In slowly growing cells, deoxyribonucleic acid synthesis was measured by autoradiography and shown to occur with greatest probability during the first two-thirds of the division cycle. In such cells, segregation occurred later, at the end of the division cycle rather than at the end of deoxyribonucleic acid replication. Nuclear regions in L-broth cells (22-min doubling time) cannot correspond to separate chromosomes but probably represent regions of replication activity. Segregation of template nucleotide strands was measured after a shift-up from proline M9 or glucose M9 media into L broth. A model is presented to account for the pattern of segregation observed.

Citing Articles

Fundamental principles in bacterial physiology-history, recent progress, and the future with focus on cell size control: a review.

Jun S, Si F, Pugatch R, Scott M Rep Prog Phys. 2018; 81(5):056601.

PMID: 29313526 PMC: 5897229. DOI: 10.1088/1361-6633/aaa628.

Threshold effect of growth rate on population variability of cell lengths.

Gangan M, Athale C R Soc Open Sci. 2017; 4(2):160417.

PMID: 28386413 PMC: 5367290. DOI: 10.1098/rsos.160417.

The bacterial nucleoid visualized by fluorescence microscopy of cells lysed within agarose: comparison of Escherichia coli and spirochetes of the genus Borrelia.

Hinnebusch B, Bendich A J Bacteriol. 1997; 179(7):2228-37.

PMID: 9079908 PMC: 178959. DOI: 10.1128/jb.179.7.2228-2237.1997.

Nucleoid partitioning and the division plane in Escherichia coli.

Woldringh C, Zaritsky A, Grover N J Bacteriol. 1994; 176(19):6030-8.

PMID: 7523361 PMC: 196821. DOI: 10.1128/jb.176.19.6030-6038.1994.

DNA segregation in Escherichia coli cells with 5-bromodeoxyuridine-substituted nucleoids.

CANOVAS J, Tresguerres E, Yousif A, Lopez-Saez J, Navarrete M J Bacteriol. 1984; 158(1):128-33.

PMID: 6370953 PMC: 215389. DOI: 10.1128/jb.158.1.128-133.1984.

References

Lark K . Regulation of chromosome replication and segregation in bacteria. Bacteriol Rev. 1966; 30(1):3-32. PMC: 378212. DOI: 10.1128/br.30.1.3-32.1966. View

Lark K, Bird R . Segregation of the conserved units of DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1965; 54(5):1444-50. PMC: 219928. DOI: 10.1073/pnas.54.5.1444. View

Lark C . Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966; 119(3):517-25. DOI: 10.1016/0005-2787(66)90128-6. View

Chai N, Lark K . Segregation of deoxyribonucleic acid in bacteria: association of the segregating unit with the cell envelope. J Bacteriol. 1967; 94(2):415-21. PMC: 315056. DOI: 10.1128/jb.94.2.415-421.1967. View

Eberle H, Lark K . Chromosome replication in Bacillus subtilis cultures growing at different rates. Proc Natl Acad Sci U S A. 1967; 57(1):95-101. PMC: 335469. DOI: 10.1073/pnas.57.1.95. View

Helmstetter C . DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. J Mol Biol. 1968; 31(3):507-18. DOI: 10.1016/0022-2836(68)90424-5. View

Cooper S, Helmstetter C . Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968; 31(3):519-40. DOI: 10.1016/0022-2836(68)90425-7. View

RYTER A . Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968; 32(1):39-54. PMC: 378291. DOI: 10.1128/br.32.1.39-54.1968. View

RYTER A, Hirota Y, Jacob F . DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968; 33:669-76. DOI: 10.1101/sqb.1968.033.01.076. View

10.

Sueoka N, Quinn W . Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968; 33:695-705. DOI: 10.1101/sqb.1968.033.01.078. View

11.

Helmstetter C, Cooper S, PIERUCCI O, Revelas E . On the bacterial life sequence. Cold Spring Harb Symp Quant Biol. 1968; 33:809-22. DOI: 10.1101/sqb.1968.033.01.093. View

12.

Bird R, Lark K . Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harb Symp Quant Biol. 1968; 33:799-808. DOI: 10.1101/sqb.1968.033.01.092. View

13.

Bird R, Lark K . Chromosome replication in Escherichia coli 15T- at different growth rates: rate of replication of the chromosome and the rate of formation of small pieces. J Mol Biol. 1970; 49(2):343-66. DOI: 10.1016/0022-2836(70)90249-4. View

14.

Powell E . Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol. 1956; 15(3):492-511. DOI: 10.1099/00221287-15-3-492. View

15.

Schaechter M, MAALOE O, KJELDGAARD N . Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958; 19(3):592-606. DOI: 10.1099/00221287-19-3-592. View

16.

RYTER A, Kellenberger E . [Embedding in polyester for ultrathin sections]. J Ultrastruct Res. 1958; 2(2):200-14. DOI: 10.1016/s0022-5320(58)90018-2. View

17.

Schaechter M, Williamson J, HOOD Jr J, Koch A . Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol. 1962; 29:421-34. DOI: 10.1099/00221287-29-3-421. View

18.

Kellenberger E, Lark K, BOLLE A . Amino acid dependent control of DNA synthesis in bacteria and vegetative phage. Proc Natl Acad Sci U S A. 1962; 48:1860-8. PMC: 221052. DOI: 10.1073/pnas.48.10.1860. View

19.

Yoshikawa H, OSullivan A, Sueoka N . SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION. Proc Natl Acad Sci U S A. 1964; 52:973-80. PMC: 300381. DOI: 10.1073/pnas.52.4.973. View