5S RNA Synthesized by Escherichia Coli in Presence of Chloramphenicol: Different 5'-terminal Sequences
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Escherichia coli cells, grown in the presence of chloramphenicol, synthesize a low molecular weight RNA (CM-5S RNA) not bound to ribosomes which is similar to ribosomal 5S RNA. Oligonucleotide patterns derived from ribonuclease digests of 5S RNA and of CM-5S RNA are indistinguishable except that the 5'-terminal oligonucleotides differ. Whereas the nucleotide sequence of the 5'-terminus of normal 5S RNA is (p)U(p)G-, there are three alternate sequences of the 5'-terminus of CM-5S RNA: (p)U(p)U(p)G-, (p)U(p)U(p)U(p)G-, and (p)A(p)U(p)U(p)U(p)G-.
One core, two shells: bacterial and eukaryotic ribosomes.
Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M Nat Struct Mol Biol. 2012; 19(6):560-7.
PMID: 22664983 DOI: 10.1038/nsmb.2313.
Nomura M Bacteriol Rev. 1970; 34(3):228-77.
PMID: 16350216 PMC: 378356. DOI: 10.1128/br.34.3.228-277.1970.
Depletion of pre-16S rRNA in starved Escherichia coli cells.
Cangelosi G, Brabant W J Bacteriol. 1997; 179(14):4457-63.
PMID: 9226253 PMC: 179279. DOI: 10.1128/jb.179.14.4457-4463.1997.
Sequence analysis and in vitro maturation of five precursor 5S RNAs from Bacillus Q.
Stiekema W, Raue H, Planta R Nucleic Acids Res. 1980; 8(10):2193-211.
PMID: 6159574 PMC: 324072. DOI: 10.1093/nar/8.10.2193.
Ribonucleic acid synthesis in bacteria treated with toluene.
Peterson R, Radcliffe C, Pace N J Bacteriol. 1971; 107(2):585-8.
PMID: 5000311 PMC: 246969. DOI: 10.1128/jb.107.2.585-588.1971.