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Corepressor System for Catabolite Repression of the Lac Operon in Escherichia Coli

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1969 Mar 1
PMID 4887497
Citations 5
Authors
W J Dobrogosz
Affiliations
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Abstract

Acetylated amino sugars, normally used in the biosynthesis of cell walls and cell membranes, were found to play a role as corepressors for catabolite repression of the lac operon in Escherichia coli. This conclusion was derived from studies conducted on mutants of E. coli that were able to assimilate an exogenous source of N-acetylglucosamine (AcGN) but were unable to dissimilate or grow on this compound. At concentrations less than 10(-4)m, AcGN caused severe catabolite repression of beta-galactosidase synthesis in cultures grown under either nonrepressed or partially repressed conditions. This repression occurred in the absence of any effect of AcGN on either the carbon and energy metabolism or the growth of the organism. In addition, this repression by AcGN occurred in a mutant strain that is constitutive for beta-galactosidase production, demonstrating that the AcGN effect does not involve the uptake of inducer. This model for the corepressor system of catabolite repression is discussed in relation to the existing theories on repression of the lac operon.

Citing Articles

Some aspects of catabolite repression of mitochondrial enzymes in Saccharomyces cerevisiae.

Gorts C Antonie Van Leeuwenhoek. 1971; 37(2):161-9.

PMID: 5314549 DOI: 10.1007/BF02218477.

Amino sugar sensitivity in Escherichia coli mutants unable to grow on N-acetylglucosamine.

Bernheim N, Dobrogosz W J Bacteriol. 1970; 101(2):384-91.

PMID: 4905307 PMC: 284918. DOI: 10.1128/jb.101.2.384-391.1970.

Catabolite repression of tryptophanase in Escherichia coli.

Botsford J, Demoss R J Bacteriol. 1971; 105(1):303-12.

PMID: 4322348 PMC: 248355. DOI: 10.1128/jb.105.1.303-312.1971.

Cyclic 3',5'-adenosine monophosphate and N-acetylglucosamine-6-phosphate as regulatory signals in catabolite repression of the lac operon in Escherichia coli.

Goldenbaum P, Broman R, Dobrogosz W J Bacteriol. 1970; 103(3):663-70.

PMID: 4319836 PMC: 248141. DOI: 10.1128/jb.103.3.663-670.1970.

Two types of glucose effects on beta-galactosidase synthesis in a membrane fraction of Escherichia coli: correlation with repression observed in intact cells.

Seto H, Nagata Y, Maruo B J Bacteriol. 1975; 122(2):660-8.

PMID: 165172 PMC: 246104. DOI: 10.1128/jb.122.2.660-668.1975.

References
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