Analysis of Melibiose Mutants Deficient in Alpha-galactosidase and Thiomethylgalactoside Permease II in Escherichia Coli K-12

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1968 Aug 1

PMID 4877127

Citations 30

Authors

R Schmitt

Affiliations

Soon will be listed here.

Abstract

Three types of mutants (mel(-)) unable to metabolize the alpha-d-galactoside, melibiose, were derived from Escherichia coli K-12. One type lacked alpha-galactosidase; another lacked a specific transport system, termed thiomethylgalactoside (TMG) permease II; and the third lacked both of these functions. The mutational sites were genetically mapped by recombination frequency with different markers and by determination of chromosomal transfer in interrupted-mating experiments. All three mel(-) mutant types mapped in a cluster near to the metA marker on the E. coli chromosome and were cotransducible. Induction studies revealed that the three alpha-d-galactosides, melibiose, melibiitol, and galactinol, induced alpha-galactosidase and TMG permease II coordinately; d-galactose also induced them but only in a galactokinaseless mutant. These data suggest that alpha-galactosidase and TMG permease II may be components of a common operon.

Citing Articles

Adaptive Evolution Compensated for the Plasmid Fitness Costs Brought by Specific Genetic Conflicts.

Li F, Wang J, Jiang Y, Guo Y, Liu N, Xiao S Pathogens. 2023; 12(1).

PMID: 36678485 PMC: 9861728. DOI: 10.3390/pathogens12010137.

Effector Overlap between the and Operons of Escherichia coli: Induction of the Operon with β-Galactosides.

Narang A, Oehler S J Bacteriol. 2017; 199(9).

PMID: 28193904 PMC: 5388812. DOI: 10.1128/JB.00796-16.

Genomics of rapid adaptation to antibiotics: convergent evolution and scalable sequence amplification.

Laehnemann D, Pena-Miller R, Rosenstiel P, Beardmore R, Jansen G, Schulenburg H Genome Biol Evol. 2014; 6(6):1287-301.

PMID: 24850796 PMC: 4079197. DOI: 10.1093/gbe/evu106.

Catabolism of raffinose, sucrose, and melibiose in Erwinia chrysanthemi 3937.

Hugouvieux-Cotte-Pattat N, Charaoui-Boukerzaza S J Bacteriol. 2009; 191(22):6960-7.

PMID: 19734309 PMC: 2772473. DOI: 10.1128/JB.00594-09.

Rcs and PhoPQ regulatory overlap in the control of Salmonella enterica virulence.

Garcia-Calderon C, Casadesus J, Ramos-Morales F J Bacteriol. 2007; 189(18):6635-44.

PMID: 17616593 PMC: 2045174. DOI: 10.1128/JB.00640-07.

References

Ganesan A, Rotman B . Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966; 16(1):42-50. DOI: 10.1016/s0022-2836(66)80261-9. View

Scaife J, Beckwith J . Mutational alteration of the maximal level of Lac operon expression. Cold Spring Harb Symp Quant Biol. 1966; 31:403-8. DOI: 10.1101/sqb.1966.031.01.052. View

Luria S, Adams J, TING R . Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology. 1960; 12:348-90. DOI: 10.1016/0042-6822(60)90161-6. View

Jacob F, MONOD J . Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961; 3:318-56. DOI: 10.1016/s0022-2836(61)80072-7. View

Cook A, LEDERBERG J . Recombination studies of lactose nonfermenting mutants of Escherichia coli K-12. Genetics. 1962; 47:1335-53. PMC: 1210286. DOI: 10.1093/genetics/47.10.1335. View

Beckwith J . A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. J Mol Biol. 1964; 8:427-30. DOI: 10.1016/s0022-2836(64)80206-0. View

Rotman B, Guzman R . Transport of galactose from the inside to the outside of Escherichia coli. Pathol Biol. 1961; 9:806-10. View

Jacob F, WOLLMAN E . Genetic and physical determinations of chromosomal segments in Escherichia coli. Symp Soc Exp Biol. 1958; 12:75-92. View

Cavalli-Sforza L . [Sexuality of bacteria]. Boll Ist Sieroter Milan. 1950; 29(9-10):281-9. View

10.

Rothman J . Transduction studies on the relation between prophage and host chromosome. J Mol Biol. 1965; 12(3):892-912. DOI: 10.1016/s0022-2836(65)80336-9. View

11.

Matney T, Goldschmidt E, ERWIN N, Scroggs R . A preliminary map of genomic sites for F-attachment in Escherichia coli K12. Biochem Biophys Res Commun. 1964; 17(3):278-81. DOI: 10.1016/0006-291x(64)90397-3. View

12.

Taylor A, Adelberg E . Linkage Analysis with Very High Frequency Males of Escherichia Coli. Genetics. 1960; 45(9):1233-43. PMC: 1210121. DOI: 10.1093/genetics/45.9.1233. View

13.

Taylor A, THOMAN M . THE GENETIC MAP OF ESCHERICHIA COLI K-12. Genetics. 1964; 50:659-77. PMC: 1210685. DOI: 10.1093/genetics/50.4.659. View

14.

LEDER I, Perry J . Galactose stimulation of beta-galactosidase induction in galactokinaseless mutants of Escherichia coli. The induction of thiomethylgalactoside permease. J Biol Chem. 1967; 242(3):457-62. View

15.

WOLLMAN E, Jacob F, Hayes W . Conjugation and genetic recombination in Escherichia coli K-12. Cold Spring Harb Symp Quant Biol. 1956; 21:141-62. DOI: 10.1101/sqb.1956.021.01.012. View

16.

Schmitt R, Rotman B . Alpha-galactosidase activity in cell-free extracts of Escherichia coli. Biochem Biophys Res Commun. 1966; 22(5):473-9. DOI: 10.1016/0006-291x(66)90297-x. View

17.

Wu H, KALCKAR H . Endogenous induction of the galactose operon in Escherichia coli K12. Proc Natl Acad Sci U S A. 1966; 55(3):622-9. PMC: 224197. DOI: 10.1073/pnas.55.3.622. View

18.

Sheppard D, ENGLESBERG E . Further evidence for positive control of the L-arabinose system by gene araC. J Mol Biol. 1967; 25(3):443-54. DOI: 10.1016/0022-2836(67)90197-0. View

19.

DEMEREC M, Adelberg E, Clark A, Hartman P . A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966; 54(1):61-76. PMC: 1211113. DOI: 10.1093/genetics/54.1.61. View

20.

Pardee A . An inducible mechanism for accumulation of melibiose in Escherichia coli. J Bacteriol. 1957; 73(3):376-85. PMC: 289808. DOI: 10.1128/jb.73.3.376-385.1957. View