Ribosome Development and the Methylation of Ribosomal Ribonucleic Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1968 May 1

PMID 4870284

Citations 7

Authors

P S Sypherd

Affiliations

Soon will be listed here.

Abstract

Immature ribonucleoprotein particles accumulate in amino acid-starved cells of a relaxed mutant of Escherichia coli. The ribonucleic acid (RNA) of these particles is nonmethylated in cells starved for methionine. However, in bacteria starved for arginine, lysine, or histidine, the RNA of these particles is one-half methylated. The relationship of submethylation to a structural alteration in the same RNA was studied. The results of kinetic studies showed that submethylation and the structural transition are not causally related, since they are described by different rate constants. Moreover, it was possible to accumulate fully methylated immature-particle RNA that possessed the sedimentation and chromatographic properties of nonmethylated RNA. It was concluded that, during the normal course of ribosome development, methylation of ribosomal RNA is completed prior to the final maturation steps.

Citing Articles

Bacterial ribosome.

Nomura M Bacteriol Rev. 1970; 34(3):228-77.

PMID: 16350216 PMC: 378356. DOI: 10.1128/br.34.3.228-277.1970.

Unique protein moieties for 30S and 50S ribosomes of Escherichia coli.

Strnad B, Sypherd P J Bacteriol. 1969; 98(3):1080-6.

PMID: 4892365 PMC: 315299. DOI: 10.1128/jb.98.3.1080-1086.1969.

Ribosomal precursors and ribonucleic acid synthesis in Escherichia coli.

Matchett W J Bacteriol. 1968; 96(4):997-1005.

PMID: 4879571 PMC: 252410. DOI: 10.1128/jb.96.4.997-1005.1968.

Nascent ribosomal and messenger RNA in DNA-membrane-complexes of Escherichia coli.

Gierer L Mol Gen Genet. 1973; 125(2):173-87.

PMID: 4590269 DOI: 10.1007/BF00268870.

Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.

Pace N Bacteriol Rev. 1973; 37(4):562-603.

PMID: 4203396 PMC: 413834. DOI: 10.1128/br.37.4.562-603.1973.

References

Sypherd P, Fansler B . Structural transitions in ribonucleic acid during ribosome development. J Bacteriol. 1967; 93(3):920-9. PMC: 276536. DOI: 10.1128/jb.93.3.920-929.1967. View

GESTELAND R . Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966; 16(1):67-84. DOI: 10.1016/s0022-2836(66)80263-2. View

Sypherd P . ACCUMULATION OF RIBONUCLEOPROTEIN PARTICLES IN A RELAXED MUTANT OF ESCHERICHIA COLI. J Bacteriol. 1965; 90:403-10. PMC: 315658. DOI: 10.1128/jb.90.2.403-410.1965. View

Sypherd P . Site of synthesis of ribosomal proteins. J Mol Biol. 1966; 17(2):358-69. DOI: 10.1016/s0022-2836(66)80147-x. View

Gordon J, Boman H . STUDIES ON MICROBIAL RNA.II. TRANSFER OF METHYL GROUPS FROM METHIONINE TO THE RNA OF A RIBONUCLEOPROTEIN PARTICLE. J Mol Biol. 1964; 9:638-53. DOI: 10.1016/s0022-2836(64)80172-8. View

Sypherd P, Strauss N . CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1963; 49(3):400-7. PMC: 299842. DOI: 10.1073/pnas.49.3.400. View

Sypherd P . FORMATION OF RIBOSOMES FROM PRECURSOR RIBONUCLEOPROTEIN PARTICLES. J Bacteriol. 1965; 90:411-7. PMC: 315659. DOI: 10.1128/jb.90.2.411-417.1965. View

DUBIN D, ELKORT A . SOME ABNORMAL PROPERTIES OF CHLORAMPHENICOL RNA. J Mol Biol. 1964; 10:508-18. DOI: 10.1016/s0022-2836(64)80069-3. View

DUBIN D . SOME EFFECT OF STREPTOMYCIN ON RNA METABOLISM IN ESCHERICHIA COLI. J Mol Biol. 1964; 8:749-67. DOI: 10.1016/s0022-2836(64)80122-4. View

10.

Manor H, Haselkorn R . Properties of ribonucleic acid components in ribonucleoprotein particle preparations obtained from an Escherichia coli RC-relaxed strain. J Mol Biol. 1967; 24(2):269-88. DOI: 10.1016/0022-2836(67)90332-4. View

11.

YANKOFSKY S, Spiegelman S . Distinct cistrons for the two ribosomal RNA components. Proc Natl Acad Sci U S A. 1963; 49:538-44. PMC: 299899. DOI: 10.1073/pnas.49.4.538. View

12.

Fleissner E, BOREK E . STUDIES ON THE ENZYMATIC METHYLATION OF SOLUBLE RNA. I. METHYLATION OF THE S-RNA POLYMER. Biochemistry. 1963; 2:1093-100. DOI: 10.1021/bi00905a032. View

13.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

14.

Britten R, McCarthy B . The synthesis of ribosomes in E. coli. II. Analysis of the kinetics of tracer incorporation in growing cells. Biophys J. 1962; 2:49-55. PMC: 1366388. DOI: 10.1016/s0006-3495(62)86840-4. View

15.

Srinivasan P, BOREK E . Enzymatic alteration of macromolecular structure. Prog Nucleic Acid Res Mol Biol. 1966; 5:157-89. DOI: 10.1016/s0079-6603(08)60234-2. View