Article: Biogenesis of beta-carotene in Mycobacterium kansasii

The biogenesis of beta-carotene in the photochromogen Mycobacterium kansasii consists of two reactions. The first reaction is photochemical, and is dependent on the wavelength of the incident light and on oxygen but is independent of temperature. The second reaction does not require illumination, and is dependent on the temperature and on oxygen. The latter, or dark reaction, requires the synthesis of new protein, and was shown to have the characteristics of an inducible system. Carotenogenesis was stimulated by incident light of wavelengths of 420, 540, and 650 nm. Immediately after illumination there was an increase in the synthesis of ribonucleic acid and beta-carotene accumulation started after a lag of 8 to 10 min. The synthesis of beta-carotene exhibited temperature dependence with an optimum of about 36 C.

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References

1.

BATRA P, Storms L . Mechanism of photoinduced and antimycin A-induced carotenoid synthesis in Mycobacterium marinum. Effect of proflavine. Biochem Biophys Res Commun. 1968; 33(5):820-7. DOI: 10.1016/0006-291x(68)90234-9. View

2.

Harding R, Huang P, MITCHELL H . Photochemical studies of the carotenoid biosynthetic pathway in Neurospora crassa. Arch Biochem Biophys. 1969; 129(2):696-707. DOI: 10.1016/0003-9861(69)90230-6. View

3.

BATRA P, Gleason Jr R, Jenkins J . Mechanism of photo-induced and antimycin A-induced carotenoid synthesis in Mycobacterium marinum. Requirements for carotenogen- esis and further evidence for protein synthesis following induction. Biochim Biophys Acta. 1969; 177(1):124-35. DOI: 10.1016/0304-4165(69)90072-5. View

4.

TARNOK I, Tarnok Z . Carotenes and xanthophylls in mycobacteria. II. Lycopene, alpha- and beta-carotene and xanthophyll in mycobacterial pigments. Tubercle. 1971; 52(2):127-35. DOI: 10.1016/0041-3879(71)90019-5. View

5.

David H . Response of Mycobacteria to ultraviolet light radiation. Am Rev Respir Dis. 1973; 108(5):1175-85. DOI: 10.1164/arrd.1973.108.5.1175. View

6.

Turian G, HAXO F . Further use of diphenylamine for the study of carotenoid biosynthesis in mycobacterium phlei. J Bacteriol. 1952; 63(5):690-1. PMC: 169328. DOI: 10.1128/jb.63.5.690-691.1952. View

7.

COHEN-BAZIRE G, STANIER R . Specific inhibition of carotenoid synthesis in a photosynthetic bacterium and its physiological consequences. Nature. 1958; 181(4604):250-2. DOI: 10.1038/181250a0. View

8.

Schlegel H . Conversion of carotenoids to oxycarotenoids by Mycobacterium phlei. J Bacteriol. 1959; 77(3):310-6. PMC: 290368. DOI: 10.1128/jb.77.3.310-316.1959. View

9.

RILLING H . Photoinduction of carotenoid synthesis of a Mycobacterium sp. Biochim Biophys Acta. 1962; 60:548-56. DOI: 10.1016/0006-3002(62)90873-9. View

10.

Kepes A . KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA. Biochim Biophys Acta. 1963; 76:293-309. View

11.

RILLING H . ON THE MECHANISM OF PHOTOINDUCTION OF CAROTENOID SYNTHESIS. Biochim Biophys Acta. 1964; 79:464-75. DOI: 10.1016/0926-6577(64)90212-8. View

12.

BATRA P, RILLING H . ON THE MECHANISM OF PHOTOINDUCED CAROTENOID SYNTHESIS: ASPECTS OF THE PHOTOINDUCTIVE REACTION. Arch Biochem Biophys. 1964; 107:485-92. DOI: 10.1016/0003-9861(64)90305-4. View

13.

RILLING H . A STUDY OF INHIBITION OF CAROTENOID SYNTHESIS. Arch Biochem Biophys. 1965; 110:39-46. DOI: 10.1016/0003-9861(65)90152-9. View

Biogenesis of Beta-carotene in Mycobacterium Kansasii