Article: Prevention of bile reflux-induced acute gastric ulceration in the rat by cholestyramine

The role of bile salts and refluxed duodenal content in the pathogenesis of acute gastric ulcers in the shocked rat were examined. The addition of cholestyramine to duodenal contents inhibits their ulcerogenic action in the stomach of rats challenged by an acid load, (100 mEq/l Hcl) and subsequently bled to a mean blood pressure of 20 mm Hg. Also, bile duct ligation 2 days before the reflux procedure and subsequent shock also inhibited ulcer formation. An ex vivo rat gastric chamber was used to further demonstrate that the "barrier" breaking action of bovine sodium taurocholate and sodium lauryl sulphate could be prevented by cholestyramine. Cholestyramine also inhibited the usual rapid depression of the transmucosal potential difference (PD) produced by both of these agents. It is concluded that cholestyramine protects the gastric mucosa from ulceration by blocking the barrier breaking action of bile salts in duodenal chyme.

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PMID: 7238276 DOI: 10.1007/BF01308106.

References

1.

Capper W . Factors in the pathogenesis of gastric ulcer. Hunterian lecture delivered at the Royal College of Surgeons of England on 18th January 1966. Ann R Coll Surg Engl. 1967; 40(1):21-35. PMC: 2311972. View

2.

COTRAN R, SUTER E, Majno G . The use of colloidal carbon as a tracer for vascular injury. A review. Vasc Dis. 1967; 4(2):107-27. View

3.

Davenport H . Destruction of the gastric mucosal barrier by detergents and urea. Gastroenterology. 1968; 54(2):175-81. View

4.

Rhodes J, Barnardo D, Phillips S, ROVELSTAD R, Hofmann A . Increased reflux of bile into the stomach in patients with gastric ulcer. Gastroenterology. 1969; 57(3):241-52. View

5.

GEALL M, Phillips S, Summerskill W . Profile of gastric potential difference in man. Effects of aspirin, alcohol, bile, and endogenous acid. Gastroenterology. 1970; 58(4):437-43. View

6.

Ivey K, Denbesten L, CLIFTON J . Effect of bile salts on ionic movement across the human gastric mucosa. Gastroenterology. 1970; 59(5):683-90. View

7.

BLACK R, Rhodes J, Davies G, Gravelle H, Sweetnam P . A controlled clinical trial of cholestyramine in the treatment of gastric ulcer. Gastroenterology. 1971; 61(6):821-5. View

8.

Hamza K, Denbesten L . Bile salts producing stress ulcers during experimental shock. Surgery. 1972; 71(2):161-7. View

9.

Denbesten L, Hamza K . Effect of bile salts on ionic permeability of canine gastric mucosa during experimental shock. Gastroenterology. 1972; 62(3):417-24. View

10.

Fisher R, Cohen S . Pyloric-sphincter dysfunction in patients with gastric ulcer. N Engl J Med. 1973; 288(6):273-6. DOI: 10.1056/NEJM197302082880601. View

11.

Braun S, Samson R, Norton L, Eiseman B . Bile reflux in experimental stress ulcer. Surgery. 1973; 73(4):521-4. View

12.

MERSEREAU W, HINCHEY E . Effect of gastric acidity on gastric ulceration induced by hemorrhage in the rat, utilizing a gastric chamber technique. Gastroenterology. 1973; 64(6):1130-5. View

13.

DUPLESSIS D . PATHOGENESIS OF GASTRIC ULCERATION. Lancet. 1965; 1(7393):974-8. DOI: 10.1016/s0140-6736(65)91214-6. View

Prevention of Bile Reflux-induced Acute Gastric Ulceration in the Rat by Cholestyramine