Article: Experience with a commercial kit for the radioisotopic assay of vitamin B12 in serum: the Phadebas B12 test

The first commercial kit for the radioisotopic assay of vitamin B(12) in serum-the Phadebas B(12) Test produced higher values than the radioisotopic method of Raven, Robson, Walker, and Barkham (1969) and the Lactobacillus leichmannii microbiological assay. Its normal range was 300-1100 pg/ml and its reproducibility was similar to that of the other radioisotopic method. It should be possible to lower the results obtained by the Phadebas method by modifying its standard curve and to reduce the time taken for the assay by shortening its incubation period.

Citing Articles

An evaluation of commercial radioisotope methods for the determination of folate and vitamin B12.

Dawson D, Delamore I, Fish D, Flaherty T, GOWENLOCK A, Hunt L J Clin Pathol. 1980; 33(3):234-42.

PMID: 7381023 PMC: 1146046. DOI: 10.1136/jcp.33.3.234.

Comparison of serum vitamin B12 estimation by saturation analysis with intrinsic factor and with R-protein as binding agents.

Zacharakis R, Muir M, Chanarin I J Clin Pathol. 1981; 34(4):357-60.

PMID: 7240422 PMC: 493290. DOI: 10.1136/jcp.34.4.357.

Interlaboratory comparison of serum vitamin B12 assay.

MOLLIN D, Hoffbrand A, WARD P, Lewis S J Clin Pathol. 1980; 33(3):243-8.

PMID: 6769970 PMC: 1146047. DOI: 10.1136/jcp.33.3.243.

Megaloblastic anaemia, cobalamin, and folate.

Chanarin I J Clin Pathol. 1987; 40(9):978-84.

PMID: 3312306 PMC: 1141167. DOI: 10.1136/jcp.40.9.978.

Extraction of serum vitamin B12 for radio-isotopic and Lactobacillus leichmannii assay.

Raven J, Robson M J Clin Pathol. 1975; 28(7):531-9.

PMID: 1150894 PMC: 475765. DOI: 10.1136/jcp.28.7.531.

References

1.

Ardeman S, Chanarin I, Krafchik B, Singer W . Addisonian pernicious anaemia and intrinsic factor antibodies in thyroid disorders. Q J Med. 1966; 35(139):421-31. View

2.

Raven J, Robson M, Walker P, BARKHAN P . The effect of cyanide, serum and other factors on the assay of vitamin B12 by a radio-isotope method using 57CoB12, intrinsic factor and coated charcoal. Guys Hosp Rep. 1968; 117(2):89-109. View

3.

Raven J, Robson M, Walker P, BARKHAN P . Improved method for measuring vitamin B12 in serum using intrinsic factor, 57CoB12, and coated charcoal. J Clin Pathol. 1969; 22(2):205-11. PMC: 474034. DOI: 10.1136/jcp.22.2.205. View

4.

HILLMAN R, Oakes M, Finholt C . Hemoglobin-coated charcoal radioassay for serum vitamin B12. A simple modification to improve intrinsic factor reliability. Blood. 1969; 34(3):385-90. View

5.

Wide L, Killander A . A radiosorbent technique for the assay of serum vitamin B12. Scand J Clin Lab Invest. 1971; 27(2):151-9. DOI: 10.3109/00365517109080202. View

6.

Raven J, Robson M, MORGAN J, Hoffbrand A . Comparison of three methods for measuring vitamin B 12 in serum: radioisotopic, euglena gracilis and Lactobacillus leichmannii. Br J Haematol. 1972; 22(1):21-31. DOI: 10.1111/j.1365-2141.1972.tb08783.x. View

7.

Kubasik N, Murray M . Comparison of two radioassay methods for vitamin B 12 . Clin Chem. 1972; 18(7):740-1. View

8.

Mortensen E . Negative interference by residual proteins in the supernatant fluid used in radioisotopic assay of serum vitamin B 12 . Clin Chem. 1972; 18(9):895-900. View

9.

Rosenthal H, SARETT H . The determination of vitamin B12 activity in human serum. J Biol Chem. 1952; 199(1):433-42. View

10.

Spray G . An improved method for the rapid estimation of vitamin B12 in serum. Clin Sci. 1955; 14(4):661-7. View

11.

Matthews D . Observations on the estimation of serum vitamin B12 using Lactobacillus leichmannii. Clin Sci. 1962; 22:101-11. View

12.

Lau K, Gottlieb C, WASSERMAN L, Herbert V . MEASUREMENT OF SERUM VITAMIN B12 LEVEL USING RADIOISOTOPE DILUTION AND COATED CHARCOAL. Blood. 1965; 26:202-14. View

Experience with a Commercial Kit for the Radioisotopic Assay of Vitamin B12 in Serum: the Phadebas B12 Test