Adenylate Cyclase in Human Gastric Mucosa: Its Activation by Histamine in Morphologically Different Biopsy Specimens

Overview

Journal Klin Wochenschr

Specialty General Medicine

Date 1979 Jul 15

PMID 470334

Citations 5

Authors

H J Ruoff

B Painz

M Becker

M Rack

K F SEWING

H Malchow

Affiliations

Soon will be listed here.

Abstract

In morphologically different biopsy specimens from fundic, antral and duodenal mucosa of 134 persons, basal and histamine stimulated adenylate cyclase activity was studied: Basal and stimulated adenylate cyclase activities were log-normally distributed. Only in the fundic but not in the antral and duodenal mucosa adenylate cyclase was sensitive to histamine. The mean basal activity in the fundic gastric mucosa was 148, in response to 10(-5) mol/l histamine 292 pmol cAMP/mg protein/20 min. In human fundic biopsy specimens histologically identified as normal gastric mucosa, the stimulatory effect of histamine on adenylate cyclase decreased with the individual's age. In bioptic material from patients suffering from histologically proven chronic gastritis the histamine effect decreased with the degree of atrophy. A similar loss of histamine sensitivity was found in gastric mucosal biopsies of antrectomized individuals operated at least 5 years before by the Billroth I or II method, whereas in the mucosa of patients with gastric or duodenal ulcer no loss occurred. In contrast, the most pronounced stimulatory action of histamine was found in this latter group. Since a histamine sensitive adenylate cyclase is localized only in the glandular area of the fundic mucosa and the histamine sensitivity depends on a morphological intact structure of the mucosa, it can be concluded, that the effects of histamine on adenylate cyclase and on hydrochloric acid acid secretion have to be considered as a mechanism linked together.

Citing Articles

Adrenergic stimulation of isolated rat gastric mucosal cells. Effect on adenylate cyclase and 14C-aminopyrine uptake.

Ruoff H, Wagner M, Gunther C, Maslinski S Naunyn Schmiedebergs Arch Pharmacol. 1982; 320(2):175-81.

PMID: 7121615 DOI: 10.1007/BF00506318.

Comparison of the effect of PGE2 and somatostatin on histamine stimulated 14C-aminopyrine uptake and cyclic AMP formation in isolated rat gastric mucosal cells.

Schepp W, Heim H, Ruoff H Agents Actions. 1983; 13(2-3):200-6.

PMID: 6135324 DOI: 10.1007/BF01967331.

Histamine-sensitive adenylate cyclase in human gastric mucosa: cellular localization and interaction by PGE2, somatostatin and secretin.

Ruoff H, Becker M Agents Actions. 1982; 12(1-2):174-5.

PMID: 6123240 DOI: 10.1007/BF01965136.

Sample taking problems in measuring actual histamine levels of human gastroduodenal mucosa: specific and general relevance in clinical trials on peptic ulcer pathogenesis and selective proximal vagotomy.

Thon K, Lorenz W, Ohmann C, Weber D, Rohde H, Roher H Gut. 1985; 26(11):1165-78.

PMID: 3905529 PMC: 1432911. DOI: 10.1136/gut.26.11.1165.

Protein kinase C inhibits stimulation of adenylate cyclase by the histamine H2 receptor in rat parietal cells.

Emly J, Hanson P Agents Actions. 1992; 37(1-2):25-9.

PMID: 1456178 DOI: 10.1007/BF01987886.

References

Martin F, MACLEOD I, Sircus W . Effects of antrectomy on the fundic mucosa of the rat. Gastroenterology. 1970; 59(3):437-44. View

Scholes P, Cooper A, Jones D, Major J, Walters M, Wilde C . Characterization of an adenylate cyclase system sensitive to histamine H2-receptor excitation in cells from dog gastric mucosa. Agents Actions. 1976; 6(6):677-82. DOI: 10.1007/BF02026087. View

Nielsen J, Thaysen E, OLESEN H, Nielsen A . Fundal gastritis after billroth-II-type resection in patients with duodenal ulcer. Scand J Gastroenterol. 1972; 7(4):337-43. DOI: 10.3109/00365527209180752. View

Capoferro R, Nygaard K . Effects of antrectomy on the gastric mucosa of the rat. Scand J Gastroenterol. 1973; 8(4):347-52. View

BLACKMAN A, Lambert D, Thayer W, MARTIN H . Computed normal values for peak acid output based on age, sex and body weight. Am J Dig Dis. 1970; 15(9):783-9. DOI: 10.1007/BF02236035. View

Kimbert D . Cyclic nucleotides and their role in gastrointestinal secretion. Gastroenterology. 1974; 67(5):1023-64. View

Troidl H, Rohde H, Lorenz W, Hafner G, Hamelmann H . Effect of selective gastric vagotomy on histamine concentration in gastric mucosa of patients with duodenal ulcer. Br J Surg. 1978; 65(1):10-6. DOI: 10.1002/bjs.1800650104. View

Wollin A, Code C, Dousa T . Interaction of prostaglandins and histamine with enzymes of cyclic AMP metabolism from guinea pig gastric mucosa. J Clin Invest. 1976; 57(6):1548-53. PMC: 436814. DOI: 10.1172/JCI108425. View

Baron J . AN ASSESSMENT OF THE AUGMENTED HISTAMINE TEST IN THE DIAGNOSIS OF PEPTIC ULCER. CORRELATIONS BETWEEN GASTRIC SECRETION, AGE AND SEX OF PATIENTS, AND SITE AND NATURE OF THE ULCER. Gut. 1963; 4:243-53. PMC: 1413442. DOI: 10.1136/gut.4.3.243. View

10.

Schmidt H, Haake U, Riecken E . [Studies on the relationship between acid secretion and parietal cell mass in gastric mucosal biopsies]. Klin Wochenschr. 1972; 50(15):744-50. DOI: 10.1007/BF01487624. View

11.

CARD W, Marks I . The relationship between the acid output of the stomach following "maximal" histamine stimulation and the parietal cell mass. Clin Sci. 1960; 19:147-63. View

12.

Ruoff H, SEWING K . Adenylate cyclase of the dog gastric mucosa: stimulation by histamine and inhibition by metiamide. Naunyn Schmiedebergs Arch Pharmacol. 1976; 294(2):207-8. DOI: 10.1007/BF00507855. View

13.

FEIFEL G, Lorenz W, Heimann A, Worsching I . [Determination of basal and maximally stimulated gastric juice secretion: critical studies on performance, evaluation and assessment of gastric secretion tests]. Klin Wochenschr. 1972; 50(8):413-22. DOI: 10.1007/BF01497568. View

14.

Jacobson E, Thompson W . Cyclic AMP and gastric secretion: the illusive second messenger. Adv Cyclic Nucleotide Res. 1976; 7:199-224. View

15.

ROHRER G, Welsh J . Correlative study: gastric secretion and histology. Gastroenterology. 1967; 52(2):185-91. View

16.

Troidl H, Lorenz W, Rohde H, Hafner G, Ronzheimer M . Histamine and peptic ulcer: a prospective study of mucosal histamine concentration in duodenal ulcer patients and in control subjects suffering from various gastrointestinal diseases. Klin Wochenschr. 1976; 54(19):947-56. DOI: 10.1007/BF01469008. View

17.

BLOOMFIELD A, Keefer C . GASTRIC ACIDITY: RELATION TO VARIOUS FACTORS SUCH AS AGE AND PHYSICAL FITNESS. J Clin Invest. 1928; 5(2):285-94. PMC: 434711. DOI: 10.1172/JCI100159. View

18.

Johnston D . A biopsy study of the gastric mucosa in postoperative patients with and without marginal ulcer. Am J Gastroenterol. 1966; 46(2):103-18. View

19.

Simon B, Czygan P, Frohling W, Kather H . Topographical studies on histamine- and adrenaline-sensitive adenylate cyclases in gastric and duodenal mucosa of human beings. Digestion. 1977; 16(1-2):185-8. DOI: 10.1159/000198071. View

20.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View