Acute Inflammatory Pulmonary Reactions Induced by Chemotactic Factors

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1979 Jul 1

PMID 464027

Citations 32

Authors

U Desai

D L Kreutzer

H Showell

C V Arroyave

P A Ward

Affiliations

Soon will be listed here.

Abstract

Acute inflammatory reactions have been produced in hamster lungs by the intrapulmonary instillation of preformed chemotactic mediators (C5fr and F-Met-Leu-Phe). By the use of 111Indium and 125Iodine labeling of homologous neutrophils (PMNs) and homologous albumin, respectively, it has been possible to obtain highly reproducible and quantitative parameters of the acute inflammatory response. The lung responds in a dose-dependent and time-dependent manner to the instillation of preformed chemotactic mediators. The quantitative parameters indicative of PMN influx were more prominent than changes in vascular permeability. The data obtained by the use of radiolabeled PMNs were confirmed by observation with light microscopy. Nonchemotactic substances such as human IgG, serum albumin, and C4 failed to induce inflammatory responses in lung. Interestingly, intact C5 instilled into lung was extremely phlogistic, apparently due to hydrolysis once within the lung. These studies provide an approach to reliable quantitative parameters of inflammatory reactions in the lung and emphasize the in vivo biologic effects of chemotactic mediators.

Citing Articles

Generation of C5a by phagocytic cells.

Huber-Lang M, Younkin E, Sarma J, Riedemann N, McGuire S, Lu K Am J Pathol. 2002; 161(5):1849-59.

PMID: 12414531 PMC: 1850785. DOI: 10.1016/S0002-9440(10)64461-6.

Requirement and role of C5a in acute lung inflammatory injury in rats.

Mulligan M, Schmid E, Beck-Schimmer B, Till G, Friedl H, Brauer R J Clin Invest. 1996; 98(2):503-12.

PMID: 8755663 PMC: 507456. DOI: 10.1172/JCI118818.

Enhanced vascular permeability and haemorrhage-inducing activity of rabbit C5ades arg: probable role of polymorphonuclear leucocyte lysosomes.

Issekutz A, Movat K, Movat H Clin Exp Immunol. 1980; 41(3):512-20.

PMID: 7438564 PMC: 1537049.

Leukocytes in chemotactic-fragment-induced lung inflammation. Vascular emigration and alveolar surface migration.

Shaw J Am J Pathol. 1980; 101(2):283-302.

PMID: 7435538 PMC: 1903602.

A differential effect of C5a and C5a des Arg in the induction of pulmonary inflammation.

Larsen G, McCarthy K, Webster R, Henson J, Henson P Am J Pathol. 1980; 100(1):179-92.

PMID: 7395964 PMC: 1903777.

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