Spore Pool Glutamic Acid As a Metabolite in Germination

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Aug 1

PMID 4626499

Citations 9

Authors

H F Foerster

Affiliations

Soon will be listed here.

Abstract

Spore glutamic acid pools were examined in dormant and germinating spores using colorimetric and (14)C analytical procedures. Germination of spores of Bacillus megaterium (parent strain), initiated by d-glucose, was accompanied by a rapid drop in the level of spore pool glutamate, from 12.0 mug/mg of dry spores to 7.7 mug/mg of dry spores after 30 sec of germination. Similar decreases in extractable spore pool glutamate were observed with l-alanine-initiated germination of B. licheniformis spores. On the other hand, glutamate pools of mutant spores of B. megaterium, with a requirement of gamma-aminobutyric acid for spore germination, remained unchanged for 9 min of germination, at which time more than 50% of the spore population had germinated. Evidence for conversion of spore pool glutamate to gamma-aminobutyric acid during germination of spores of B. megaterium (parent strain) was obtained.

Citing Articles

Cloning of the gene for glutamate decarboxylase and its expression during conidiation in Neurospora crassa.

Hao R, Schmit J Biochem J. 1993; 293 ( Pt 3):735-8.

PMID: 8352739 PMC: 1134427. DOI: 10.1042/bj2930735.

Regulation and glutamic acid decarboxylase during Neurospora crassa conidial germination.

CHRISTENSEN R, Schmit J J Bacteriol. 1980; 144(3):983-90.

PMID: 6449504 PMC: 294761. DOI: 10.1128/jb.144.3.983-990.1980.

Regulation of aconitase synthesis in Bacillus subtilis: induction, feedback repression, and catabolite repression.

Ohne M J Bacteriol. 1974; 117(3):1295-305.

PMID: 4205196 PMC: 246613. DOI: 10.1128/jb.117.3.1295-1305.1974.

Glutamic acid decarboxylase in spores of Bacillus megaterium and its possible involvement in spore germination.

Foerster C, Foerster H J Bacteriol. 1973; 114(3):1090-8.

PMID: 4197264 PMC: 285369. DOI: 10.1128/jb.114.3.1090-1098.1973.

Repression of sporulation in Bacillus subtilis by L-malate.

Ohne M, RUTBERG B J Bacteriol. 1976; 125(2):453-60.

PMID: 812866 PMC: 236103. DOI: 10.1128/jb.125.2.453-460.1976.

References

Foerster H, FOSTER J . Response of Bacillus spores to combinations of germinative compounds. J Bacteriol. 1966; 91(3):1168-77. PMC: 316010. DOI: 10.1128/jb.91.3.1168-1177.1966. View

HYATT M, LEVINSON H . Correlation of respiratory activity with phases of spore germination and growth in Bacillus megaterium as influenced by manganese and L-alanine. J Bacteriol. 1956; 72(2):176-83. PMC: 357875. DOI: 10.1128/jb.72.2.176-183.1956. View

Cozzani I, Misuri A, Santoni C . Purification and general properties of glutamate decarboxylase from Clostridium perfringens. Biochem J. 1970; 118(1):135-41. PMC: 1179089. DOI: 10.1042/bj1180135. View

Mandels G, LEVINSON H, HYATT M . Analysis of respiration during germination and enlargement of spores of Bacillus megaterium and of the fungus Myrothecium verrucaria. J Gen Physiol. 1956; 39(3):301-9. PMC: 2147541. DOI: 10.1085/jgp.39.3.301. View

LEVINSON H, HYATT M . The stimulation of germination and respiration of Bacillus megaterium spores by mangasese, L-alanine and heat. J Bacteriol. 1955; 70(4):368-74. PMC: 386235. DOI: 10.1128/jb.70.4.368-374.1955. View

OConnor R, HALVORSON H . Intermediate metabolism of aerobic spores. IV. Alanine deamination during the germination of spores of Bacillus cereus. J Bacteriol. 1959; 78:844-51. PMC: 290642. DOI: 10.1128/jb.78.6.844-851.1959. View

Rosen H . A modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys. 1957; 67(1):10-5. DOI: 10.1016/0003-9861(57)90241-2. View

Setlow P, Kornberg A . Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination. J Biol Chem. 1970; 245(14):3645-52. View

Nelson D, Kornberg A . Biochemical studies of bacterial sporulation and germination. 18. Free amino acids in spores. J Biol Chem. 1970; 245(5):1128-36. View

10.

Foerster H . -aminobutyric acid as a required germinant for mutant spores of Bacillus megaterium. J Bacteriol. 1971; 108(2):817-23. PMC: 247146. DOI: 10.1128/jb.108.2.817-823.1971. View

11.

Strausbauch P, FISCHER E . Chemical and physical properties of Escherichia coli glutamate decarboxylase. Biochemistry. 1970; 9(2):226-33. DOI: 10.1021/bi00804a006. View

12.

Moore S, STEIN W . Chromatography of amino acids on sulfonated polystyrene resins. J Biol Chem. 1951; 192(2):663-81. View

13.

Setlow P, Kornberg A . Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores. J Biol Chem. 1970; 245(14):3637-44. View

14.

Akers E, Aronson J . Detection on polyacrylamide gels of L-glutamic acid decarboxylase activities from Bacillus thuringiensis. Anal Biochem. 1971; 39(2):535-8. DOI: 10.1016/0003-2697(71)90446-5. View

15.

Powell J, STRANGE R . Biochemical changes occurring during the germination of bacterial spores. Biochem J. 1953; 54(2):205-9. PMC: 1268925. DOI: 10.1042/bj0540205. View