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Metachromatic Agar-diffusion Microslide Technique for Detecting Staphylococcal Nuclease in Foods

Overview
Journal Appl Microbiol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1972 Jan 1
PMID 4621793
Citations 8
Authors
R V Lachica
P D Hoeprich
C Genigeorgis
Affiliations
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Abstract

The metachromatic agar-diffusion (MAD) microslide technique was shown to detect nanogram quantities of staphylococcal thermonuclease in various foods without prior extraction, purification, or concentration.

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References
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CHESBRO W, Auborn K . Enzymatic detection of the growth of Staphylococcus aureus in foods. Appl Microbiol. 1967; 15(5):1150-9. PMC: 547157. DOI: 10.1128/am.15.5.1150-1159.1967. View
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Victor R, Lachica F, Weiss K, Deibel R . Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus. Appl Microbiol. 1969; 18(1):126-7. PMC: 377910. DOI: 10.1128/am.18.1.126-127.1969. View
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Lachica R, Deibel R . Detection of nuclease activity in semisolid and broth cultures. Appl Microbiol. 1969; 18(2):174-6. PMC: 377938. DOI: 10.1128/am.18.2.174-176.1969. View
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Lachica R, Hoeprich P, Genigeorgis C . Nuclease production and lysostaphin susceptibility of Staphylococcus aureus and other catalase-positive cocci. Appl Microbiol. 1971; 21(5):823-6. PMC: 377289. DOI: 10.1128/am.21.5.823-826.1971. View