Isolation of Membrane-associated Folded Chromosomes from Escherichia Coli: Effect of Protein Synthesis Inhibition

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Dec 1

PMID 4612018

Citations 17

Authors

O A Ryder

D W Smith

Affiliations

Soon will be listed here.

Abstract

The sedimentation properties of membrane-associated folded chromosomes prepared from Escherichia coli TAU-bar at 0 to 4 C were studied. Utilizing a modification of the procedure of Stonington and Pettijohn (1971), quantitative yields of membrane-associated folded chromosomes may be obtained. Folded chromosomes remained associated with the cell envelope during their replication and after completion of residual synthesis in the absence of required amino acids, as demonstrated by sedimentation velocities and the presence of high levels of cosedimenting protein. Membrane-associated folded chromosomes isolated from amino acid-starved cells sedimented more rapidly than membrane-associated folded chromosomes isolated from exponentially growing cells.

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Electron microscopy of membrane-free folded chromosomes from Escherichia coli.

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Envelope-associated folded chromosomes for Escherichia coli: variations under different physiological conditions.

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References

BILLEN D, Hewitt R . Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J Bacteriol. 1966; 92(3):609-17. PMC: 276298. DOI: 10.1128/jb.92.3.609-617.1966. View

Morgan C, Rosenkranz H, CARR H, Rose H . Electron microscopy of chloramphenicol-treated Escherichia coli. J Bacteriol. 1967; 93(6):1987-2002. PMC: 276719. DOI: 10.1128/jb.93.6.1987-2002.1967. View

Bird R, Lark K . Initiation and termination of DNA replication after amino acid starvation of E. coli 15T-. Cold Spring Harb Symp Quant Biol. 1968; 33:799-808. DOI: 10.1101/sqb.1968.033.01.092. View

KUEMPEL P . Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli. J Bacteriol. 1969; 100(3):1302-10. PMC: 250320. DOI: 10.1128/jb.100.3.1302-1310.1969. View

Caro L, Berg C . Chromosome replication in Escherichia coli. II. Origin of replication in F- and F+ strains. J Mol Biol. 1969; 45(2):325-36. DOI: 10.1016/0022-2836(69)90108-9. View

Hirota Y, Mordoh J, Jacob F . On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation. J Mol Biol. 1970; 53(3):369-87. DOI: 10.1016/0022-2836(70)90072-0. View

STONINGTON O, PETTIJOHN D . The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proc Natl Acad Sci U S A. 1971; 68(1):6-9. PMC: 391088. DOI: 10.1073/pnas.68.1.6. View

Carl P . Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet. 1970; 109(2):107-22. DOI: 10.1007/BF00269647. View

Wechsler J, Gross J . Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971; 113(3):273-84. DOI: 10.1007/BF00339547. View

10.

WORCEL A, Burgi E . On the structure of the folded chromosome of Escherichia coli. J Mol Biol. 1972; 71(2):127-47. DOI: 10.1016/0022-2836(72)90342-7. View

11.

Schubach W, Whitmer J, Davern C . Genetic control of DNA initiation in Escherichia coli. J Mol Biol. 1973; 74(2):205-21. DOI: 10.1016/0022-2836(73)90107-1. View

12.

Marunouchi T, Messer W . Replication of a specific terminal chromosome segment in Escherichia coli which is required for cell division. J Mol Biol. 1973; 78(1):211-28. DOI: 10.1016/0022-2836(73)90439-7. View

13.

Dworsky P, Schaechter M . Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli. J Bacteriol. 1973; 116(3):1364-74. PMC: 246496. DOI: 10.1128/jb.116.3.1364-1374.1973. View

14.

Delius H, WORCEL A . Letter: Electron microscopic visualization of the folded chromosome of Escherichia coli. J Mol Biol. 1974; 82(1):107-9. DOI: 10.1016/0022-2836(74)90577-4. View

15.

MAALOE O, HANAWALT P . Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961; 3:144-55. DOI: 10.1016/s0022-2836(61)80041-7. View

16.

HANAWALT P . Involvement of synthesis of RNA in thymineless death. Nature. 1963; 198:286. DOI: 10.1038/198286a0. View

17.

Davis J, SINSHEIMER R . The replication of bacteriophage MS2. 1. Transfer of parental nucleic acid to progeny phage. J Mol Biol. 1963; 6:203-7. DOI: 10.1016/s0022-2836(63)80069-8. View

18.

Lark K, Repko T, Hoffman E . THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963; 76:9-24. View

19.

Cummings D . SEDIMENTATION AND BIOLOGICAL PROPERTIES OF T-PHAGES OF ESCHERICHIA COLI. Virology. 1964; 23:408-18. DOI: 10.1016/0042-6822(64)90264-8. View

20.

Lark C, Lark K . EVIDENCE FOR TWO DISTINCT ASPECTS OF THE MECHANISM REGULATING CHROMOSOME REPLICATION IN ESCHERICHIA COLI. J Mol Biol. 1964; 10:120-36. DOI: 10.1016/s0022-2836(64)80032-2. View