Determination of Bacteroides Melaninogenicus Serogroups by Fluorescent Antibody Staining

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Oct 1

PMID 4609033

Citations 20

Authors

D W Lambe Jr

Affiliations

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Abstract

Fluorescein isothiocyanate-labeled antibody reagents (conjugates) were prepared to one strain of each of the three subspecies of Bacteroides melaninogenicus: B. melaninogenicus subsp. melaninogenicus, B. melaninogenicus subsp. asaccharolyticus, and B. melaninogenicus subsp. intermedius. These three conjugates were specific; thus, they provided a new serological classification of B. melaninogenicus. The three serogroups were designated A, B, and C. Most test strains (98%) isolated from human clinical specimens were assigned to a specific serogroup by immunofluorescence, and the serogroup of these test strains corroborated the biochemical characterization of the three subspecies of B. melaninogenicus. The conjugates failed to cross-react with other anaerobes or aerobes tested. This fluorescent antibody technique provided a more rapid classification of the three subspecies of B. melaninogenicus than did conventional biochemical methods.

Citing Articles

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Cellular distribution and linkage of D-(-)-3-hydroxy fatty acids in Bacteroides species.

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Comparison of antisera in the fluorescent antibody test for detection of Bacteroides spp in clinical specimens.

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References

Rosan B . Antigens of Streptococcus sanguis. Infect Immun. 1973; 7(2):205-11. PMC: 422661. DOI: 10.1128/iai.7.2.205-211.1973. View

Altemeier W . THE BACTERIAL FLORA OF ACUTE PERFORATED APPENDICITIS WITH PERITONITIS: A BACTERIOLOGIC STUDY BASED UPON ONE HUNDRED CASES. Ann Surg. 1938; 107(4):517-28. PMC: 1386835. DOI: 10.1097/00000658-193804000-00006. View

Werner H, Sebald M . [Serological study of gram-negative sporeless anaerobes, and especially of bacteroides convexus and bacteroides melaninogenicus]. Ann Inst Pasteur (Paris). 1968; 115(3):350-66. View

NOBLES Jr E . Bacteroides infections. Ann Surg. 1973; 177(5):601-6. PMC: 1355605. View

COURANT P, Gibbons R . Biochemical and immunological heterogeneity of Bacteroides melaninogenicus. Arch Oral Biol. 1967; 12(12):1605-13. DOI: 10.1016/0003-9969(67)90194-x. View

Sabbaj J, Sutter V, Finegold S . Anaerobic pyogenic liver abscess. Ann Intern Med. 1972; 77(4):627-38. DOI: 10.7326/0003-4819-77-4-629. View

Sawyer S, Macdonald J, Gibbons R . Biochemical characteristics of Bacteroides melaninogenicus. A study of thirty-one strains. Arch Oral Biol. 1962; 7:685-91. DOI: 10.1016/0003-9969(62)90117-6. View

KEREBEL B, SEDALLIAN A . [Effect of Bacteroides melaninogenicus on dentin in vitro]. SSO Schweiz Monatsschr Zahnheilkd. 1972; 82(7):731-4. View

Gibbons R, Socransky S, Sawyer S, KAPSIMALIS B, Macdonald J . The microbiota of the gingival crevice area of man. II. The predominant cultivable organisms. Arch Oral Biol. 1963; 8:281-9. DOI: 10.1016/0003-9969(63)90020-7. View

10.

Socransky S, Gibbons R, Dale A, BORTNICK L, Rosenthal E, Macdonald J . The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Arch Oral Biol. 1963; 8:275-80. DOI: 10.1016/0003-9969(63)90019-0. View

11.

Griffin M . Fluorescent antibody techniques in the identification of the gram-negative nonsporeforming anaerobes. Health Lab Sci. 1970; 7(2):78-83. View

12.

Felner J, Dowell Jr V . "Bacteroides" bacteremia. Am J Med. 1971; 50(6):787-96. DOI: 10.1016/0002-9343(71)90187-2. View