Article: Nalidixic acid for enrichment of auxotrophs in cultures of Salmonella typhimurium

Nalidixic acid kills only growing cells of Salmonella typhimurium and can be used to enrich for auxotrophs in populations where prototrophs predominate.

Citing Articles

Characterization of a Marine Bacterium Associated with Crassostrea virginica (the Eastern Oyster).

Weiner R, Segall A, Colwell R Appl Environ Microbiol. 1985; 49(1):83-90.

PMID: 16346712 PMC: 238349. DOI: 10.1128/aem.49.1.83-90.1985.

Isolation and characterization of spontaneously arising auxotrophic and Kanagawa phenomenon-negative mutants of Vibrio parahaemolyticus.

Burstyn D, McNicol L, VOLL M Infect Immun. 1980; 27(3):889-96.

PMID: 7380557 PMC: 550858. DOI: 10.1128/iai.27.3.889-896.1980.

Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.

Lengeler J Mol Gen Genet. 1980; 179(1):49-54.

PMID: 6450313 DOI: 10.1007/BF00268445.

Transductional construction of a threonine-hyperproducing strain of Serratia marcescens: lack of feedback controls of three aspartokinases and two homoserine dehydrogenases.

Komatsubara S, KISUMI M, Chibata I Appl Environ Microbiol. 1983; 45(5):1445-52.

PMID: 6307143 PMC: 242483. DOI: 10.1128/aem.45.5.1445-1452.1983.

Genetic and physical characterization of proBA genes of the marine bacterium Vibrio parahaemolyticus.

Datta A, Ostroff R, MACQUILLAN A Appl Environ Microbiol. 1987; 53(12):2733-8.

PMID: 2829719 PMC: 204189. DOI: 10.1128/aem.53.12.2733-2738.1987.

References

1.

Hartman P, Hartman Z, STAHL R . Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella. Adv Genet. 1971; 16:1-34. DOI: 10.1016/s0065-2660(08)60352-1. View

2.

Ramareddy G, Reiter H . Specific loss of newly replicated deoxyribonucleic acid in nalidixic acid-treated Bacillus subtilis 168. J Bacteriol. 1969; 100(2):724-9. PMC: 250150. DOI: 10.1128/jb.100.2.724-729.1969. View

3.

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

4.

Goss W, Deitz W, Cook T . MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI. J Bacteriol. 1964; 88:1112-8. PMC: 314861. DOI: 10.1128/jb.88.4.1112-1118.1964. View

5.

Cook T, Deitz W, Goss W . Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J Bacteriol. 1966; 91(2):774-9. PMC: 314928. DOI: 10.1128/jb.91.2.774-779.1966. View

6.

TAKETO A, Watanabe H . Effect of nalidixic acid on the growth of bacterial viruses. J Biochem. 1967; 61(4):520-2. DOI: 10.1093/oxfordjournals.jbchem.a128579. View

7.

Goss W, Deitz W, Cook T . MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965; 89:1068-74. PMC: 277597. DOI: 10.1128/jb.89.4.1068-1074.1965. View

8.

Ishida T, Seto S, Osawa T . Use of dihydrostreptomycin for the isolation of auxotrophic mutants of Pseudomonas aeruginosa. J Bacteriol. 1966; 91(3):1387. PMC: 316046. DOI: 10.1128/jb.91.3.1387-.1966. View

9.

Davis B . The Isolation of Biochemically Deficient Mutants of Bacteria by Means of Penicillin. Proc Natl Acad Sci U S A. 1949; 35(1):1-10. PMC: 1062948. DOI: 10.1073/pnas.35.1.1. View

10.

Cook T, Goss W, Deitz W . Mechanism of Action of Nalidixic Acid on Escherichia coli V. Possible Mutagenic Effect. J Bacteriol. 1966; 91(2):780-3. PMC: 314929. DOI: 10.1128/jb.91.2.780-783.1966. View

11.

Deitz W, Cook T, Goss W . Mechanism of action of nalidixic acid on Escherichia coli. 3. Conditions required for lethality. J Bacteriol. 1966; 91(2):768-73. PMC: 314927. DOI: 10.1128/jb.91.2.768-773.1966. View

Nalidixic Acid for Enrichment of Auxotrophs in Cultures of Salmonella Typhimurium