Sequence of Events in Initiation of Translation: a Role for Initiator Transfer RNA in the Recognition of Messenger RNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Aug 1

PMID 4607060

Citations 9

Authors

G Jay

R KAEMPFER

Affiliations

Soon will be listed here.

Abstract

It is shown that initiation of translation involves several steps. (i) Binding of fMet-tRNA(fMet) to the bacterial 30S ribosomal subunit in the absence of messenger RNA, yielding a 34S complex. This binding is rapid and dependent on initiation factor 2 but not on initiation factor 3. (ii) Binding of messenger RNA to the 34S complex. This binding is slower and depends on initiation factor 3. If R17 RNA is used as messenger, the resulting complex sediments at 46 S. (iii) Joining of a 50S subunit to yield a complete initiation complex. Binding of fMet-tRNA(fMet) not only precedes, but is necessary for, correct binding of messenger RNA to ribosomes. Thus, initiator tRNA may play an active role in the selection of initiation sites in messenger RNA.

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