Proteins at the TRNA Binding Sites of Escherichia Coli Ribosomes

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 Jan 1

PMID 4589893

Citations 14

Authors

A P Czernilofsky

E E Collatz

G Stoffler

E Kuechler

Affiliations

Soon will be listed here.

Abstract

p - Nitrophenylcarbamyl - phenylalanyl-tRNA binds to ribosomes in response to poly(U) and reacts with ribosomal proteins via covalent bond formation. Ribosomal proteins of Escherichia coli were characterized by two-dimensional gel electrophoresis and by reaction with specific antibodies. The affinity label is shown to react with the 50S proteins L27, L15, L2, L16, and L14. We therefore conclude that these particular proteins are located at or near tRNA-binding sites within the 50S ribosomal subunit.

Citing Articles

A new simpler photoaffinity analogue of peptidyl tRNA.

Hsiung N, Cantor C Nucleic Acids Res. 1974; 1(12):1753-62.

PMID: 4616224 PMC: 343454. DOI: 10.1093/nar/1.12.1753.

Photo-affinity labeling of tRNA binding sites in macromolecules. I. Linking of the phenacyl-p-azide of 4-thiouridine in (Escherichia coli) valyl-tRNA to 16S RNA at the ribosomal P site.

Schwartz I, OFENGAND J Proc Natl Acad Sci U S A. 1974; 71(10):3951-5.

PMID: 4610566 PMC: 434304. DOI: 10.1073/pnas.71.10.3951.

A kinase that transfers the gamma-phosphoryl group of GTP to proteins of eukaryotic 40S ribosomal subunits.

Ventimiglia F, Wool I Proc Natl Acad Sci U S A. 1974; 71(2):350-4.

PMID: 4521807 PMC: 388002. DOI: 10.1073/pnas.71.2.350.

Localization of ribosomal protein L27 at the peptidyl transferase centre of the 50 S subunit, as determined by immuno-electron microscopy.

Lotti M, Stoffler-Meilicke M, Stoffler G Mol Gen Genet. 1987; 210(3):498-503.

PMID: 3123891 DOI: 10.1007/BF00327203.

Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.

Bolotin-Fukuhara M Nucleic Acids Res. 1988; 16(19):9299-306.

PMID: 3050898 PMC: 338707. DOI: 10.1093/nar/16.19.9299.

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