Purification of Single- and Double-length M13 Virions by Polyacrylamide Gel Electrophoresis

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1974 Feb 1

PMID 4589859

Citations 7

Authors

J Beaudoin

T J Henry

D Pratt

Affiliations

Soon will be listed here.

Abstract

Complete separation of single- and double-length M13 virions from each other and from still-longer phage particles was achieved by electrophoresis of phage stocks through 2.5% polyacrylamide gels.

Citing Articles

Improvements in the production of purified M13 bacteriophage bio-nanoparticle.

Passaretti P, Khan I, Dafforn T, Goldberg Oppenheimer P Sci Rep. 2020; 10(1):18538.

PMID: 33122639 PMC: 7596064. DOI: 10.1038/s41598-020-75205-3.

Carbon nanotube thin film biosensors for sensitive and reproducible whole virus detection.

Mandal H, Su Z, Ward A, Shirley Tang X Theranostics. 2012; 2(3):251-7.

PMID: 22448194 PMC: 3311235. DOI: 10.7150/thno.3726.

Analysis of promoter mutations in the histidine transport operon of Salmonella typhimurium: use of hybrid M13 bacteriophages for cloning, transformation, and sequencing.

Lee G, AMES G J Bacteriol. 1984; 159(3):1000-5.

PMID: 6090381 PMC: 215759. DOI: 10.1128/jb.159.3.1000-1005.1984.

Double-length, circular, single-stranded DNA from filamentous phage.

WHEELER F, Benzinger R, Bujard H J Virol. 1974; 14(3):620-30.

PMID: 4604346 PMC: 355557. DOI: 10.1128/JVI.14.3.620-630.1974.

Antiserum inactivation of electrophoretically purified M13 diploid virions: model for the F-specific filamentous bacteriophages.

Beaudoin J, Pratt D J Virol. 1974; 13(2):466-9.

PMID: 4589858 PMC: 355317. DOI: 10.1128/JVI.13.2.466-469.1974.

References

Hjerten S, JERSTEDT S, TISELIUS A . Some aspects of the use of "continuous" and "discontinuous" buffer systems in polyacrylamide gel electrophoresis. Anal Biochem. 1965; 11(2):219-23. DOI: 10.1016/0003-2697(65)90008-4. View

Arber W, Morse M . HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA COLI. VI. EFFECTS ON BACTERIAL CONJUGATION. Genetics. 1965; 51:137-48. PMC: 1210762. DOI: 10.1093/genetics/51.1.137. View

Pattee P . Use of tetrazolium for improved resolution of bacteriophage plaques. J Bacteriol. 1966; 92(3):787-8. PMC: 276324. DOI: 10.1128/jb.92.3.787-788.1966. View

SALIVAR W, Henry T, Pratt D . Purification and properties of diploid particles of coliphage M13. Virology. 1967; 32(1):41-51. DOI: 10.1016/0042-6822(67)90250-4. View

Caro L, Schnos M . The attachment of the male-specific bacteriophage F1 to sensitive strains of Escherichia coli. Proc Natl Acad Sci U S A. 1966; 56(1):126-32. PMC: 285685. DOI: 10.1073/pnas.56.1.126. View

Benzinger R . Restriction of infectious bacteriophage fd DNA's and an assay for in vitro host-controlled restriction and modification. Proc Natl Acad Sci U S A. 1968; 59(4):1294-9. PMC: 224866. DOI: 10.1073/pnas.59.4.1294. View

Marvin D, Hohn B . Filamentous bacterial viruses. Bacteriol Rev. 1969; 33(2):172-209. PMC: 378320. DOI: 10.1128/br.33.2.172-209.1969. View

Pratt D, TZAGOLOFF H, Beaudoin J . Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins. Virology. 1969; 39(1):42-53. DOI: 10.1016/0042-6822(69)90346-8. View

Lang D, Mitani M . Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970; 9(3):373-9. DOI: 10.1002/bip.1970.360090310. View

10.

Wiseman R, Dunker A, Marvin D . Filamentous bacterial viruses. 3. Physical and chemical characterization of the If1 virion. Virology. 1972; 48(1):230-44. DOI: 10.1016/0042-6822(72)90130-4. View

11.

SIMON E . The distribution and significance of multiploid virus particles. Prog Med Virol. 1972; 14:36-67. View

12.

Beaudoin J, Pratt D . Antiserum inactivation of electrophoretically purified M13 diploid virions: model for the F-specific filamentous bacteriophages. J Virol. 1974; 13(2):466-9. PMC: 355317. DOI: 10.1128/JVI.13.2.466-469.1974. View

13.

Kellenberger E, Arber W . Electron microscopical studies of phage multiplication. I. A method for quantitative analysis of particle suspensions. Virology. 1957; 3(2):245-55. DOI: 10.1016/0042-6822(57)90091-0. View

14.

BRADLEY D . THE STRUCTURE OF SOME BACTERIOPHAGES ASSOCIATED WITH MALE STRAINS OF ESCHERICHIA COLI. J Gen Microbiol. 1964; 35:471-82. DOI: 10.1099/00221287-35-3-471. View

15.

SALIVAR W, TZAGOLOFF H, Pratt D . SOME PHYSICAL-CHEMICAL AND BIOLOGICAL PROPERTIES OF THE ROD-SHAPED COLIPHAGE M13. Virology. 1964; 24:359-71. DOI: 10.1016/0042-6822(64)90173-4. View

16.

Pratt D, TZAGOLOFF H, Erdahl W . Conditional lethal mutants of the small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action. Virology. 1966; 30(3):397-410. DOI: 10.1016/0042-6822(66)90118-8. View